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Denitration of 2,4,6-trinitrotoluene by Pseudomonas aeruginosa ESA-5 in the presence of ferrihydrite.

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Eyers, Laurent [UCL] Stenuit, Benoît [UCL] Agathos, Spiros N. [UCL]

Denitration of 2,4,6-trinitrotoluene (TNT) was evaluated in oxygen-depleted enrichment cultures. These cultures were established starting with an uncontaminated or a TNT-contaminated soil inoculum and contained TNT as sole nitrogen source. Incubations were carried out in the presence or absence of ferrihydrite. A significant release of nitrite was observed in the liquid culture containing TNT, ferrihydrite, and inoculum from a TNT-contaminated soil. Under these conditions, Pseudomonas aeruginosa was the predominant bacterium in the enrichment, leading to the isolation of P. aeruginosa ESA-5 as a pure strain. The isolate had TNT denitration capabilities as confirmed by nitrite release in oxygen-depleted cultures containing TNT and ferrihydrite. In addition to reduced derivatives of TNT, several unidentified metabolites were detected. Concomitant to a decrease of TNT concentration, a release of nitrite was observed. The concentration of nitrite peaked and then it slowly decreased. In the absence of TNT, the drop in the concentration of nitrite in oxygen-depleted cultures was lower when ferrihydrite was provided, suggesting that ferrihydrite inhibited the utilization of nitrite by P. aeruginosa ESA-5.

Document type Article de périodique (Journal article) – Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2008
Journal information "Applied Microbiology and Biotechnology" - Vol. 79, no. 3, p. 489-497 (2008)
Peer reviewed yes
Publisher Springer ((Germany) Heidelberg)
issn 0175-7598
e-issn 1432-0614
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Anaerobiosis ; Biodegradation, Environmental ; Chromatography, High Pressure Liquid ; DNA Fingerprinting ; Ferric Compounds - metabolism ; Nitrites - metabolism ; Pseudomonas aeruginosa - chemistry - genetics - isolation & purification - metabolism ; Soil Microbiology ; Trinitrotoluene - analysis - metabolism
Links
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Bibliographic reference Eyers, Laurent ; Stenuit, Benoît ; Agathos, Spiros N.. Denitration of 2,4,6-trinitrotoluene by Pseudomonas aeruginosa ESA-5 in the presence of ferrihydrite.. In: Applied Microbiology and Biotechnology, Vol. 79, no. 3, p. 489-497 (2008)
Permanent URL http://hdl.handle.net/2078.1/23077