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Complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cDNA clone.

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Darville, M I Crepin, K M Vandekerckhove, J. Van Damme, J. [Laboratory of Genetics, Rijksuniversiteit, B-9000 Gent, Belgium] Octave, Jean-Noël [UCL] Rider, Mark H. [UCL] Marchand, Maurice [UCL] Hue, Louis [UCL] Rousseau, Guy [UCL]

cDNA clones for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were isolated from rat liver expression libraries in lambda gt11 by antibody, oligonucleotide, and cDNA screening. One 1860 bp long clone contained a full-length nucleotide sequence coding for the 470 amino acids of each of the two identical subunits of the bifunctional enzyme. This clone also contained untranslated sequences, one 173 bp long upstream from the ATG start codon and one 271 bp long downstream from the TGA stop codon. The clone was terminated by a poly(A) tail of 29 nucleotides.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1987
Journal information "FEBS letters" - Vol. 224, no. 2, p. 317-21 (1987)
Peer reviewed yes
issn 0014-5793
Publication status Publié
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
MESH Subject Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; DNA - genetics ; Liver - enzymology ; Molecular Sequence Data ; Phosphofructokinase-2 ; Phosphoric Monoester Hydrolases - genetics ; Phosphotransferases (Alcohol Group Acceptor) - genetics ; Rats
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Bibliographic reference Darville, M I ; Crepin, K M ; Vandekerckhove, J. ; Van Damme, J. ; Octave, Jean-Noël ; et. al. Complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cDNA clone.. In: FEBS letters, Vol. 224, no. 2, p. 317-21 (1987)
Permanent URL http://hdl.handle.net/2078.1/21644