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Sp1 can displace GHF-1 from its distal binding site and stimulate transcription from the growth hormone gene promoter

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Lemaigre, Frédéric [UCL] Lafontaine, D A Courtois, S J Durviaux, S M Rousseau, Guy [UCL]

DNase I footprinting experiments showed that binding activities of Sp1 and of GHF-1 to its distal site on the human growth hormone gene promoter are mutually exclusive. The kinetics of GHF-1 binding were indicative of positive cooperativity. The Sp1 site did not affect promoter activity in cell-free transcription. Still, Sp1 could compensate partially for the decreased stimulation of transcription seen at low GHF-1 concentrations.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès libre
Publication date 1990
Journal information "Molecular and Cellular Biology" - Vol. 10, no. 4, p. 1811-4 (1990)
Peer reviewed yes
issn 0270-7306
Publication status Publié
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
MESH Subject Base Sequence ; Binding, Competitive ; DNA-Binding Proteins - metabolism ; Deoxyribonuclease I ; Genes ; Growth Hormone - genetics ; Humans ; Kinetics ; Molecular Sequence Data ; Pituitary Gland, Anterior ; Promoter Regions, Genetic ; Restriction Mapping ; Sp1 Transcription Factor ; Transcription Factors - metabolism ; Transcription, Genetic
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Bibliographic reference Lemaigre, Frédéric ; Lafontaine, D A ; Courtois, S J ; Durviaux, S M ; Rousseau, Guy. Sp1 can displace GHF-1 from its distal binding site and stimulate transcription from the growth hormone gene promoter. In: Molecular and Cellular Biology, Vol. 10, no. 4, p. 1811-4 (1990)
Permanent URL http://hdl.handle.net/2078.1/21608