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Isoforms of hepatocyte nuclear factor-6 differ in DNA-binding properties, contain a bifunctional homeodomain, and define the new ONECUT class of homeodomain proteins

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Lannoy, V J Bürglin, T R Rousseau, Guy [UCL] Lemaigre, Frédéric [UCL]

Hepatocyte nuclear factor-6 (HNF-6) contains a single cut domain and a homeodomain characterized by a phenylalanine at position 48 and a methionine at position 50. We describe here two isoforms of HNF-6 which differ by the linker that separates these domains. Both isoforms stimulated transcription. The affinity of HNF-6alpha and HNF-6beta for DNA differed, depending on the target sequence. Binding of HNF-6 to DNA involved the cut domain and the homeodomain, but the latter was not required for binding to a subset of sites. Mutations of the F48M50 dyad that did not affect DNA binding reduced the transcriptional stimulation of constructs that do not require the homeodomain for DNA binding, but did not affect the stimulation of constructs that do require the homeodomain. Comparative trees of mammalian, Drosophila, and Caenorhabditis elegans proteins showed that HNF-6 defines a new class, which we call ONECUT, of homeodomain proteins. C. elegans proteins of this class bound to HNF-6 DNA targets. Thus, depending on their sequence, these targets determine for HNF-6 at least two modes of DNA binding, which hinge on the homeodomain and on the linker that separates it from the cut domain, and two modes of transcriptional stimulation, which hinge on the homeodomain.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1998
Journal information "Journal of Biological Chemistry" - Vol. 273, no. 22, p. 13552-62 (1998)
Peer reviewed yes
Publisher American Society for Biochemistry and Molecular Biology, Inc.
issn 1083-351X
Publication status Publié
Affiliation UCL - MD/BICL - Département de biochimie et de biologie cellulaire
MESH Subject Amino Acid Sequence ; Amino Acid Substitution ; Animals ; Base Sequence ; DNA - metabolism ; DNA Primers ; DNA-Binding Proteins - chemistry - metabolism ; Hepatocyte Nuclear Factor 6 ; Homeodomain Proteins - chemistry - metabolism ; Isomerism ; Methionine - chemistry - metabolism ; Molecular Sequence Data ; Phenylalanine - chemistry - metabolism ; Protein Binding ; Rats ; Sequence Homology, Amino Acid ; Trans-Activators - chemistry - metabolism ; Transcriptional Activation
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Bibliographic reference Lannoy, V J ; Bürglin, T R ; Rousseau, Guy ; Lemaigre, Frédéric. Isoforms of hepatocyte nuclear factor-6 differ in DNA-binding properties, contain a bifunctional homeodomain, and define the new ONECUT class of homeodomain proteins. In: Journal of Biological Chemistry, Vol. 273, no. 22, p. 13552-62 (1998)
Permanent URL http://hdl.handle.net/2078.1/21598