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Characterization of a ring-hydroxylating dioxygenase from phenanthrene-degrading Sphingomonas sp. strain LH128 able to oxidize benz[a]anthracene.

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Schuler, Luc [UCL] Jouanneau, Yves [CEA, iRTSV, Laboratoire de Chimie et Biologie des Métaux and UMR 5249 CNRS, Université J. Fourier, 38054 Grenoble Cedex 9, France] Ní Chadhain, Sinéad [Rutgers - The State University of New Jersey] Meyer, Christine [CEA, iRTSV, Laboratoire de Chimie et Biologie des Métaux and UMR 5249 CNRS, Université J. Fourier, 38054 Grenoble Cedex 9, France] Pouli, Maria [UCL] Hols, Pascal [UCL] Agathos, Spiros N. [UCL]

Sphingomonas sp. strain LH128 was isolated from a polycyclic aromatic hydrocarbon (PAH)-contaminated soil using phenanthrene as the sole source of carbon and energy. A dioxygenase complex, phnA1fA2f, encoding the alpha and beta subunit of a terminal dioxygenase responsible for the initial attack on PAHs, was identified and isolated from this strain. PhnA1f showed 98%, 78%, and 78% identity to the alpha subunit of PAH dioxygenase from Novosphingobium aromaticivorans strain F199, Sphingomonas sp. strain CHY-1, and Sphingobium yanoikuyae strain B1, respectively. When overexpressed in Escherichia coli, PhnA1fA2f was able to oxidize low-molecular-weight PAHs, chlorinated biphenyls, dibenzo-p-dioxin, and the high-molecular-weight PAHs benz[a]anthracene, chrysene, and pyrene. The action of PhnA1fA2f on benz[a]anthracene produced two benz[a]anthracene dihydrodiols.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2009
Language Anglais
Journal information "Applied Microbiology and Biotechnology" - Vol. 83, no. 3, p. 465-475 (2009)
Peer reviewed yes
Publisher Springer ((Germany) Heidelberg)
issn 0175-7598
e-issn 1432-0614
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Substrate Specificity ; Sphingomonas - chemistry - enzymology - genetics - isolation & purification ; Soil Microbiology ; Polycyclic Hydrocarbons, Aromatic - metabolism ; Phenanthrenes - metabolism ; Molecular Sequence Data ; Dioxygenases - chemistry - genetics - metabolism ; Biodegradation, Environmental ; Benz(a)Anthracenes - metabolism ; Bacterial Proteins - chemistry - genetics - metabolism
Links
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Bibliographic reference Schuler, Luc ; Jouanneau, Yves ; Ní Chadhain, Sinéad ; Meyer, Christine ; Pouli, Maria ; et. al. Characterization of a ring-hydroxylating dioxygenase from phenanthrene-degrading Sphingomonas sp. strain LH128 able to oxidize benz[a]anthracene.. In: Applied Microbiology and Biotechnology, Vol. 83, no. 3, p. 465-475 (2009)
Permanent URL http://hdl.handle.net/2078.1/21328