User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Community composition of arbuscular mycorrhizal fungi associated with native plants growing in a petroleum-polluted soil of the Amazon region of Ecuador

Primary tabs

download
  • Open access
  • PDF
  • 550.80 K
Garcés‐Ruiz, Mónica [Laboratorio de micología, Carrera de Microbiología, Facultad de Ciencia Exactas y Naturales, Pontificia Universidad Católica del Ecuador, Quito, Ecuador] Senés-Guerrero, Carolina [Tecnologico de Monterrey, Campus Guadalajara, Av. General Ramón Corona 2514, Nuevo México, CP 45138, Zapopan, Jal., México] Declerck, Stephan [UCL] Cranenbrouck, Sylvie [UCL]

Arbuscular mycorrhizal fungi (AMF) are worldwide distributed plant symbionts. However, their occurrence in hydrocarbon-polluted environments is less investigated, although specific communities may be present with possible interest for remediation strategies. Here, we investigated the AMF community composition associated with the roots of diverse plant species naturally recolonizing a weathered crude oil pond in the Amazon region of Ecuador. Next generation 454 GS-Junior sequencing of an 800 bp LSU rRNA gene PCR amplicon was used. PCR amplicons were affiliated to a maximum-likelihood phylogenetic tree computed from 1.5 kb AMF reference sequences. A high throughput phylogenetic annotation approach, using an evolutionary placement algorithm (EPA) allowed the characterization of sequences to the species level. Fifteen species were detected. Acaulospora species were identified as dominant colonizers, with 73% of relative read abundance, Archaeospora (19.6%) and several genera from the Glomeraceae (Rhizophagus, Glomus macrocarpum-like, Sclerocystis, Dominikia and Kamienskia) were also detected. Although, a diverse community belonging to Glomeraceae was revealed, they represented <10% of the relative abundance in the Pond. Seventy five % of the species could not be identified, suggesting possible new species associated with roots of plants under highly hydrocarbon-polluted conditions.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2019
Language Anglais
Journal information "MicrobiologyOpen" - p. e00703 (2019)
Peer reviewed yes
Publisher John Wiley & Sons, Inc. (Bognor Regis)
issn 2045-8827
e-issn 2045-8827
Publication status Accepté/Sous presse
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Keywords Microbiology ; 454-pyrosequencing ; Arbuscular mycorrhizal fungi ; Ecuador ; amazonian soil ; community composition ; hydrocarbon ; polluted environment
Links
  1. Bioremediation applied microbial solutions for real-world environmental cleanup (2005)
  2. Berger Simon A., Krompass Denis, Stamatakis Alexandros, Performance, Accuracy, and Web Server for Evolutionary Placement of Short Sequence Reads under Maximum Likelihood, 10.1093/sysbio/syr010
  3. Berger Simon A., Stamatakis Alexandros, Aligning short reads to reference alignments and trees, 10.1093/bioinformatics/btr320
  4. Błaszkowski Janusz, Chwat Gerard, Góralska Anna, Ryszka Przemysław, Kovács Gábor M., Two new genera, Dominikia and Kamienskia, and D. disticha sp. nov. in Glomeromycota, 10.1127/nova_hedwigia/2014/0216
  5. Błaszkowski, Mycotaxon, 76, 187 (2000)
  6. Brundrett Mark C., Tedersoo Leho, Evolutionary history of mycorrhizal symbioses and global host plant diversity, 10.1111/nph.14976
  7. Cabello, Nova Hedwigia, 73, 513 (2001)
  8. Caporaso, Nature Publishing Group, 7, 335 (2010)
  9. Chagnon Pierre-Luc, Bradley Robert L., Maherali Hafiz, Klironomos John N., A trait-based framework to understand life history of mycorrhizal fungi, 10.1016/j.tplants.2013.05.001
  10. Conesa A., Gotz S., Garcia-Gomez J. M., Terol J., Talon M., Robles M., Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research, 10.1093/bioinformatics/bti610
  11. de la Providencia Ivan E., Stefani Franck O.P., Labridy Manuel, St-Arnaud Marc, Hijri Mohamed, Arbuscular mycorrhizal fungal diversity associated with Eleocharis obtusa and Panicum capillare growing in an extreme petroleum hydrocarbon-polluted sedimentation basin, 10.1093/femsle/fnv081
  12. Garcés-Ruiz Mónica, Senés-Guerrero Carolina, Declerck Stéphane, Cranenbrouck Sylvie, Arbuscular Mycorrhizal Fungal Community Composition in Carludovica palmata, Costus scaber and Euterpe precatoria from Weathered Oil Ponds in the Ecuadorian Amazon, 10.3389/fmicb.2017.02134
  13. Hassan Saad El-Din, Bell Terrence H., Stefani Franck O. P., Denis David, Hijri Mohamed, St-Arnaud Marc, Contrasting the Community Structure of Arbuscular Mycorrhizal Fungi from Hydrocarbon-Contaminated and Uncontaminated Soils following Willow (Salix spp. L.) Planting, 10.1371/journal.pone.0102838
  14. Huang, Chinese Journal of Ecology., 2007, 14 (2007)
  15. Iffis Bachir, St-Arnaud Marc, Hijri Mohamed, Petroleum hydrocarbon contamination, plant identity and arbuscular mycorrhizal fungal (AMF) community determine assemblages of the AMF spore-associated microbes : Assemblages of AMF and their spore-associated microbes, 10.1111/1462-2920.13438
  16. Krüger Manuela, Krüger Claudia, Walker Christopher, Stockinger Herbert, Schüßler Arthur, Phylogenetic reference data for systematics and phylotaxonomy of arbuscular mycorrhizal fungi from phylum to species level, 10.1111/j.1469-8137.2011.03962.x
  17. Krüger Manuela, Stockinger Herbert, Krüger Claudia, Schüßler Arthur, DNA-based species level detection ofGlomeromycota: one PCR primer set for all arbuscular mycorrhizal fungi, 10.1111/j.1469-8137.2009.02835.x
  18. Labana Sumeet, Kapur Manisha, Malik Deepak K., Prakash Dhan, Jain R.K., Diversity, Biodegradation and Bioremediation of Polycyclic Aromatic Hydrocarbons, Environmental Bioremediation Technologies ISBN:9783540347903 p.409-443, 10.1007/978-3-540-34793-4_18
  19. Lee, Phytoremediation for Green Energy, 1 (2015)
  20. Lenoir Ingrid, Fontaine Joël, Lounès-Hadj Sahraoui Anissa, Arbuscular mycorrhizal fungal responses to abiotic stresses: A review, 10.1016/j.phytochem.2016.01.002
  21. Lenoir I., Lounes-Hadj Sahraoui A., Fontaine J., Arbuscular mycorrhizal fungal-assisted phytoremediation of soil contaminated with persistent organic pollutants: a review, 10.1111/ejss.12375
  22. Liu Aiguo, Dalpé Yolande, REDUCTION IN SOIL POLYCYCLIC AROMATIC HYDROCARBONS BY ARBUSCULAR MYCORRHIZAL LEEK PLANTS, 10.1080/15226510802363444
  23. Loján Paul, Senés-Guerrero Carolina, Suárez Juan Pablo, Kromann Peter, Schüßler Arthur, Declerck Stéphane, Potato field-inoculation in Ecuador with Rhizophagus irregularis: no impact on growth performance and associated arbuscular mycorrhizal fungal communities, 10.1007/s13199-016-0471-2
  24. McGONIGLE T. P., MILLER M. H., EVANS D. G., FAIRCHILD G. L., SWAN J. A., A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi, 10.1111/j.1469-8137.1990.tb00476.x
  25. Ministerio del Ambiente de Ecuador (MAE), Pasivos ambientales y reparación integral: Experiencias de gestión en el Ecuador (2016)
  26. Morton Joseph B., Three New Species of Acaulospora (Endogonaceae) from High Aluminum, Low pH Soils in West Virginia, 10.2307/3807777
  27. Oksanen , J Blanchet , F. G. Friendly , M. l. Kindt , R. Legendre , P. McGlinn , D. Wagner , H. 2017 http://cran.r-project.org/package=vegan
  28. Phytoremediation for Green Energy, ISBN:9789400778863, 10.1007/978-94-007-7887-0
  29. Rajtor Monika, Piotrowska-Seget Zofia, Prospects for arbuscular mycorrhizal fungi (AMF) to assist in phytoremediation of soil hydrocarbon contaminants, 10.1016/j.chemosphere.2016.07.071
  30. Sahoo Naresh K., Ramesh Aiyagari, Pakshirajan Kannan, Bacterial Degradation of Aromatic Xenobiotic Compounds: An Overview on Metabolic Pathways and Molecular Approaches, Microorganisms in Environmental Management (2012) ISBN:9789400722286 p.201-220, 10.1007/978-94-007-2229-3_10
  31. Microorganisms in environmental management (2012)
  32. Senés-Guerrero Carolina, Schüßler Arthur, A conserved arbuscular mycorrhizal fungal core-species community colonizes potato roots in the Andes, 10.1007/s13225-015-0328-7
  33. Senés-Guerrero Carolina, Schüßler Arthur, DNA-Based Characterization and Identification of Arbuscular Mycorrhizal Fungi Species, Microbial Environmental Genomics (MEG) (2016) ISBN:9781493933679 p.101-123, 10.1007/978-1-4939-3369-3_6
  34. Senés-Guerrero Carolina, Torres-Cortés Gloria, Pfeiffer Stefan, Rojas Mercy, Schüßler Arthur, Potato-associated arbuscular mycorrhizal fungal communities in the Peruvian Andes, 10.1007/s00572-013-0549-0
  35. Smith, Mycorrhizal symbiosis (2008)
  36. Standard Methods for the Examination of Water and Wastewater SM 4500-N 1997 http://www.standardmethods.org/store/ProductView.cfm?ProductID=194
  37. Standard Methods for the Examination of Water and Wastewater SM5520 E 2012 https://www.standardmethods.org/store/ProductView.cfm?ProductID=41
  38. Stockinger Herbert, Krüger Manuela, Schüßler Arthur, DNA barcoding of arbuscular mycorrhizal fungi, 10.1111/j.1469-8137.2010.03262.x
  39. Stutz Jean C., Morton Joseph B., Successive pot cultures reveal high species richness of arbuscular endomycorrhizal fungi in arid ecosystems, 10.1139/b96-225
  40. Tang M., Chen H., Huang J.C., Tian Z.Q., AM fungi effects on the growth and physiology of Zea mays seedlings under diesel stress, 10.1016/j.soilbio.2008.11.007
  41. Team RDC 2016 http://www.r-project.org/
  42. Walker, Inoculum, 56, 68 (2005)
  43. Wu F.Y., Yu X.Z., Wu S.C., Lin X.G., Wong M.H., Phenanthrene and pyrene uptake by arbuscular mycorrhizal maize and their dissipation in soil, 10.1016/j.jhazmat.2011.01.024
Bibliographic reference Garcés‐Ruiz, Mónica ; Senés-Guerrero, Carolina ; Declerck, Stephan ; Cranenbrouck, Sylvie. Community composition of arbuscular mycorrhizal fungi associated with native plants growing in a petroleum-polluted soil of the Amazon region of Ecuador. In: MicrobiologyOpen, p. e00703 (2019)
Permanent URL http://hdl.handle.net/2078.1/209690