Vierra, Nicholas C
Dadi, Prasanna K
Milian, Sarah C
Dickerson, Matthew T
Jordan, Kelli L
Gilon, Patrick
[UCL]
Jacobson, David A
Ca2+ handling by the endoplasmic reticulum (ER) serves critical roles in controlling pancreatic β cell function and becomes perturbed during the pathogenesis of diabetes. ER Ca2+ homeostasis is determined by ion movements across the ER membrane, including K+ flux through K+ channels. We demonstrated that K+ flux through ER-localized TALK-1 channels facilitated Ca2+ release from the ER in mouse and human β cells. We found that β cells from mice lacking TALK-1 exhibited reduced basal cytosolic Ca2+ and increased ER Ca2+ concentrations, suggesting reduced ER Ca2+ leak. These changes in Ca2+ homeostasis were presumably due to TALK-1-mediated ER K+ flux, because we recorded K+ currents mediated by functional TALK-1 channels on the nuclear membrane, which is continuous with the ER. Moreover, overexpression of K+-impermeable TALK-1 channels in HEK293 cells did not reduce ER Ca2+ stores. Reduced ER Ca2+ content in β cells is associated with ER stress and islet dysfunction in diabetes, and islets from TALK-1-deficient mice fed a high-fat diet showed reduced signs of ER stress, suggesting that TALK-1 activity exacerbated ER stress. Our data establish TALK-1 channels as key regulators of β cell ER Ca2+ and suggest that TALK-1 may be a therapeutic target to reduce ER Ca2+ handling defects in β cells during the pathogenesis of diabetes.
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Bibliographic reference |
Vierra, Nicholas C ; Dadi, Prasanna K ; Milian, Sarah C ; Dickerson, Matthew T ; Jordan, Kelli L ; et. al. TALK-1 channels control β cell endoplasmic reticulum Ca2+ homeostasis.. In: Science Signaling, Vol. 10, p. eaan2883 (2017) |
Permanent URL |
http://hdl.handle.net/2078.1/193975 |