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Diversity and enzymatic potentialities of Bacillus sp. strains isolated from a polluted freshwater ecosystem in Cuba

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Larrea Murrell, Jeny Adina [UCL] Rojas-Badia, Marcia Maria [Laboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty Havana University Havana Cuba] García-Soto, Ivette [Laboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty Havana University Havana Cuba] Romeu-Alvarez, Beatriz [Laboratory of Microbial Ecology, Microbiology and Virology Department, Biology Faculty Havana University Havana Cuba] Bacchetti, Tristano [Instituto Madrileño de Estudios Avanzados Agua (IMDEA Agua). C/Punto Net 4Parque Científico Tecnológico de la Universidad de AlcaláAlcalá de HenaresSpain] Gillis, Annika [UCL] Mahillon, Jacques [UCL]

Genotypic and phenotypic characterization of Bacillus spp. from polluted freshwater has been poorly addressed. The objective of this research was to determine the diversity and enzymatic potentialities of Bacillus spp. strains isolated from the Almendares River. Bacilli strains from a polluted river were characterized by considering the production of extracellular enzymes using API ZYM. 14 strains were selected and identified using 16S rRNA, gyrB and aroE genes. Genotypic diversity of the Bacillus spp. strains was evaluated using pulsed field gel electrophoresis. Furthermore, the presence of genetic determinants of potential virulence toxins of the Bacillus cereus group and proteinaceous crystal inclusions of Bacillus thuringiensis was determined. 10 strains were identified as B. thuringiensis, two as Bacillus megaterium, one as Bacillus pumilus and one as Bacillus subtilis. Most strains produced proteases, amylases, phosphatases, esterases, aminopeptidases and glucanases, which reflect the abundance of biopolymeric matter in Almendares River. Comparison of the typing results revealed a spatio-temporal distribution among B. thuringiensis strains along the river. The results of the present study highlight the genotypic and phenotypic diversity of Bacillus spp. strains from a polluted river, which contributes to the knowledge of genetic diversity of Bacilli from tropical polluted freshwater ecosystems.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2018
Language Anglais
Journal information "World Journal of Microbiology and Biotechnology" - Vol. 34, p. 28 (2018)
Peer reviewed yes
Publisher Springer Netherlands (Dordrecht)
issn 0959-3993
e-issn 1573-0972
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Keywords Biotechnology ; Applied Microbiology and Biotechnology ; Physiology ; General Medicine ; Bacillus spp. ; Bacillus thuringiensis ; Enzyme activity ; Freshwater ; Polluted river
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Bibliographic reference Larrea Murrell, Jeny Adina ; Rojas-Badia, Marcia Maria ; García-Soto, Ivette ; Romeu-Alvarez, Beatriz ; Bacchetti, Tristano ; et. al. Diversity and enzymatic potentialities of Bacillus sp. strains isolated from a polluted freshwater ecosystem in Cuba. In: World Journal of Microbiology and Biotechnology, Vol. 34, p. 28 (2018)
Permanent URL http://hdl.handle.net/2078.1/193508