Marcélis, Lionel
[UCL]
Surin, Mathieu
Lartia, Rémy
Moucheron, Cécile
Defrancq, Eric
Kirsch-De Mesmaeker, Andrée
Under irradiation, Ru–TAP (TAP = 1,4,5,8-tetraazaphenanthrene) complexes tethered to oligonucleotides (Ru–ODN) hybridized to their complementary target sequences produce irreversible adducts with guanine (G) bases. The interesting feature is that the G bases that react in the double strand (DS) topology belong exclusively to the complementary strand; thus, no G base of the Ru–ODN strand participates in the production of these adducts. This highly specific photoreaction in the DS topology is demonstrated by gel electrophoresis analyses of different duplex sequences in which the positions of the G bases are varied. Atomistic molecular modelling simulations indicate that this specificity is due to important steric constraints for the intrastrand adduct formation in the duplex. Luminescence lifetime measurements furnish more detailed information on these different duplex systems.
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- Olofsson Johan, Önfelt Björn, Lincoln Per, Three-State Light Switch of [Ru(phen)2dppz]2+: Distinct Excited-State Species with Two, One, or No Hydrogen Bonds from Solvent, 10.1021/jp037967k
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- Mei H. Y., Barton J. K., Tris(tetramethylphenanthroline)ruthenium(II): a chiral probe that cleaves A-DNA conformations., 10.1073/pnas.85.5.1339
- Ruggi Albert, van Leeuwen Fijs W.B., Velders Aldrik H., Interaction of dioxygen with the electronic excited state of Ir(III) and Ru(II) complexes: Principles and biomedical applications, 10.1016/j.ccr.2011.05.012
- Delaney Sarah, Pascaly Matthias, Bhattacharya Pratip K., Han Koun, Barton Jacqueline K., Oxidative Damage by Ruthenium Complexes Containing the Dipyridophenazine Ligand or Its Derivatives: A Focus on Intercalation, 10.1021/ic0111738
- Barton Jacqueline K., Olmon Eric D., Sontz Pamela A., Metal complexes for DNA-mediated charge transport, 10.1016/j.ccr.2010.09.002
- Elias Benjamin, Kirsch-De Mesmaeker Andrée, Photo-reduction of polyazaaromatic Ru(II) complexes by biomolecules and possible applications, 10.1016/j.ccr.2005.11.011
- Marcélis Lionel, Ghesquière Jonathan, Garnir Kevin, Kirsch-De Mesmaeker Andrée, Moucheron Cécile, Photo-oxidizing RuII complexes and light: Targeting biomolecules via photoadditions, 10.1016/j.ccr.2012.02.012
- Ghesquiere Jonathan, Le Gac Stephane, Marcelis Lionel, Moucheron Cecile, Kirsch - De Mesmaeker Andree, What Does the Future Hold for Photo-Oxidizing RuII Complexes with Polyazaaromatic Ligands in Medicinal Chemistry?, 10.2174/156802612799079008
- Marcelis L., Moucheron C., Kirsch-De Mesmaeker A., Ru-TAP complexes and DNA: from photo-induced electron transfer to gene photo-silencing in living cells, 10.1098/rsta.2012.0131
- Jacquet Luc, Kelly John M., Mesmaeker Andrée Kirsch-De, Photoadduct between tris(1,4,5,8-tetraazaphenanthrene)ruthenium(II) and guanosine monophosphate–a model for a new mode of covalent binding of metal complexes to DNA, 10.1039/c39950000913
- Reschner A, Bontems S, Le Gac S, Lambermont J, Marcélis L, Defrancq E, Hubert P, Moucheron C, Kirsch-De Mesmaeker A, Raes M, Piette J, Delvenne P, Ruthenium oligonucleotides, targeting HPV16 E6 oncogene, inhibit the growth of cervical cancer cells under illumination by a mechanism involving p53, 10.1038/gt.2012.54
- Le Gac Stéphane, Rickling Stéphane, Gerbaux Pascal, Defrancq Eric, Moucheron Cécile, Kirsch-De Mesmaeker Andrée, A Photoreactive Ruthenium(II) Complex Tethered to a Guanine-Containing Oligonucleotide: A Biomolecular Tool that Behaves as a “Seppuku Molecule”, 10.1002/anie.200804503
- Le Gac Stéphane, Surin Mathieu, Defrancq Eric, Moucheron Cécile, Kirsch-De Mesmaeker Andrée, What Are the Parameters Controlling Inter- vs. Intra-Strand DNA Photodamage with Ru-TAP Oligonucleotides?, 10.1002/ejic.201201019
- Villien Mathilde, Deroo Stéphanie, Gicquel Etienne, Defrancq Eric, Moucheron Cécile, Kirsch-De Mesmaeker Andrée, Dumy Pascal, The oxime bond formation as an efficient tool for the conjugation of ruthenium complexes to oligonucleotides and peptides, 10.1016/j.tet.2007.08.088
- Deroo Stéphanie, Defrancq Eric, Moucheron Cécile, Kirsch-De Mesmaeker Andrée, Dumy Pascal, Synthesis of an oxyamino-containing phenanthroline derivative for the efficient preparation of phenanthroline oligonucleotide oxime conjugates, 10.1016/j.tetlet.2003.09.128
- Im Wonpil, Lee Michael S., Brooks Charles L., Generalized born model with a simple smoothing function, 10.1002/jcc.10321
Bibliographic reference |
Marcélis, Lionel ; Surin, Mathieu ; Lartia, Rémy ; Moucheron, Cécile ; Defrancq, Eric ; et. al. Specificity of Light-Induced Covalent Adduct Formation between RuIIOligonucleotide Conjugates and Target Sequences for Gene Silencing Applications : Light-Induced Covalent Adducts for Gene Silencing Applications. In: European Journal of Inorganic Chemistry, Vol. 2014, p. 3016-3022 (2014) |
Permanent URL |
http://hdl.handle.net/2078/192628 |