User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

A new approach for diagnosing chronic myelomonocytic leukemia using structural parameters of Sysmex XNTM analyzers in routine laboratory practice.

Primary tabs

download
  • Open access
  • PDF
  • 0.99 M
Schillinger, Françoise Sourdeau, Elise Boubaya, Marouane Baseggio, Lucile Clauser, Sylvain Defour, Jean-Philippe [UCL] Mullier, François [UCL] Deconinck, Eric

According to WHO recommendations, diagnosis of chronic myelomonocytic leukemia (CMML) beforehand requires microscopic examination of peripheral blood to identify dysplasia and/or blasts when monocytes are greater or equal to 1.0 × 109/L and 10% of leucocytes. We analyzed parameters derived from SysmexTM XN analyzers to improve the management of microscopic examination for monocytosis. We analyzed results of the complete blood count and the positioning and dispersion parameters of polymorphonuclear neutrophils and monocytes in 61 patients presenting with CMML and 635 control patients presenting with a reactive monocytosis. We used logistic regression and multivariate analysis to define a score for smear review. Three parameters were selected: neutrophil/monocyte ratio, structural neutrophil dispersion (Ne-WX) and monocyte absolute value. We established an equation in which the threshold of 0.160 guided microscopic examination in the search for CMML abnormalities with a sensitivity of 0.967 and a specificity of 0.978 in the learning cohort (696 samples) and 0.923 and 0.936 in the validation cohort (1809 samples) respectively. We created a score for microscopic smear examination of patients presenting with a monocytosis greater or equal to 1.0 × 109/L and 10% of leucocytes, improving efficiency in laboratory routine practice.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2018
Language Anglais
Journal information "Scandinavian Journal of Clinical & Laboratory Investigation" - Vol. 78, no. 3, p. 159-164 (2018)
Peer reviewed yes
Publisher Informa Healthcare ((United Kingdom) London)
issn 0036-5513
e-issn 1502-7686
Publication status Publié
Affiliations UCL - SSS/DDUV - Institut de Duve
UCL - SSS/DDUV/SIGN - Cell signalling
UCL - SSS/IREC/MONT - Pôle Mont Godinne
UCL - (MGD) Laboratoire de biologie clinique
UCL - (SLuc) Service de biologie hématologique
Keywords Hematology ; Automated blood count ; Chronic myelomonocytic leukemia ; Disease diagnostics ; Microscopy
Links
  1. Maynadie M., Girodon F., Manivet-Janoray I., Mounier M., Mugneret F., Bailly F., Favre B., Caillot D., Petrella T., Flesch M., Carli P.-M., Twenty-five years of epidemiological recording on myeloid malignancies: data from the specialized registry of hematologic malignancies of Cote d'Or (Burgundy, France), 10.3324/haematol.2010.026252
  2. Guru Murthy Guru Subramanian, Dhakal Ishwori, Mehta Paulette, Incidence and survival outcomes of chronic myelomonocytic leukemia in the United States, 10.1080/10428194.2016.1258700
  3. Dinmohamed Avinash G., van Norden Yvette, Visser Otto, Posthuma Eduardus F.M., Huijgens Peter C., Sonneveld Pieter, van de Loosdrecht Arjan A., Jongen-Lavrencic Mojca, The use of medical claims to assess incidence, diagnostic procedures and initial treatment of myelodysplastic syndromes and chronic myelomonocytic leukemia in the Netherlands, 10.1016/j.leukres.2014.11.025
  4. Swerdlow SH, WHO classification of tumours of haematopoietic and lymphoid tissues (2008)
  5. Vardiman J. W., Thiele J., Arber D. A., Brunning R. D., Borowitz M. J., Porwit A., Harris N. L., Le Beau M. M., Hellstrom-Lindberg E., Tefferi A., Bloomfield C. D., The 2008 revision of the World Health Organization (WHO) classification of myeloid neoplasms and acute leukemia: rationale and important changes, 10.1182/blood-2009-03-209262
  6. Arber D. A., Orazi A., Hasserjian R., Thiele J., Borowitz M. J., Le Beau M. M., Bloomfield C. D., Cazzola M., Vardiman J. W., The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia, 10.1182/blood-2016-03-643544
  7. McCullough Kristen B., Patnaik Mrinal M., Chronic Myelomonocytic Leukemia: a Genetic and Clinical Update, 10.1007/s11899-015-0271-4
  8. Patnaik M M, Tefferi A, Cytogenetic and molecular abnormalities in chronic myelomonocytic leukemia, 10.1038/bcj.2016.5
  9. Elena C., Galli A., Such E., Meggendorfer M., Germing U., Rizzo E., Cervera J., Molteni E., Fasan A., Schuler E., Ambaglio I., Lopez-Pavia M., Zibellini S., Kuendgen A., Travaglino E., Sancho-Tello R., Catricala S., Vicente A. I., Haferlach T., Haferlach C., Sanz G. F., Malcovati L., Cazzola M., Integrating clinical features and genetic lesions in the risk assessment of patients with chronic myelomonocytic leukemia, 10.1182/blood-2016-05-714030
  10. Patnaik Mrinal M., Tefferi Ayalew, Chronic myelomonocytic leukemia: 2016 update on diagnosis, risk stratification, and management : Chronic Myelomonocytic Leukemia, 10.1002/ajh.24396
  11. Parikh Sameer A., Tefferi Ayalew, Chronic myelomonocytic leukemia: 2013 update on diagnosis, risk stratification, and management : Annual Clinical Updates in Hematological Malignancies: A Continuing Medical Education Series, 10.1002/ajh.23574
  12. Bacher Ulrike, Haferlach Torsten, Schnittger Susanne, Kreipe Hans, Kröger Nicolaus, Recent advances in diagnosis, molecular pathology and therapy of chronic myelomonocytic leukaemia : Review, 10.1111/j.1365-2141.2011.08631.x
  13. Cornet Edouard, Mullier François, Despas Noemie, Jacqmin Hugues, Geara Carole, Boubaya Marouane, Chatelain Bernard, Troussard Xavier, Evaluation and optimization of theextendedinformation process unit (E-IPU) validation module integrating the sysmex flag systems and the recommendations of the French-speaking cellular hematology group (GFHC), 10.1080/00365513.2016.1199049
  14. Genevieve F, Feuillets De Biologie, 317 (2014)
  15. Miguel Amparo, Orero Maite, Simon Ramon, Collado Rosa, Perez Pedro L, Pacios Alejandro, Iglesias Rosario, Martinez Antonio, Carbonell Felix, Automated Neutrophil Morphology and Its Utility in the Assessment of Neutrophil Dysplasia, 10.1532/lh96.07011
  16. Inaba Tohru, Yuki Yoichi, Yuasa Soichi, Fujita Naohisa, Yoshitomi Kazue, Kamisako Toshinori, Torii Kunio, Okada Toshiharu, Urasaki Yoshimasa, Ueda Takanori, Tohyama Kaoru, Clinical utility of the neutrophil distribution pattern obtained using the CELL-DYN SAPPHIRE hematology analyzer for the diagnosis of myelodysplastic syndrome, 10.1007/s12185-011-0892-x
  17. LE ROUX G., VLAD A., ECLACHE V., MALANQUIN C., COLLON J.-F., GANTIER M., SCHILLINGER F., PELTIER J.-Y., SAVIN B., LETESTU R., BARAN-MARSZAK F., FENAUX P., AJCHENBAUM-CYMBALISTA F., Routine diagnostic procedures of myelodysplastic syndromes: value of a structural blood cell parameter (NEUT-X) determined by the Sysmex XE-2100TM : ROUTINE DIAGNOSTIC PROCEDURES OF MYELODYSPLASTIC SYNDROMES, 10.1111/j.1751-553x.2010.01247.x
  18. Boutault R, Blood, 128, 5518 (2016)
  19. Shen Qi, Ouyang Juan, Tang Guilin, Jabbour Elias J., Garcia-Manero Guillermo, Routbort Mark, Konoplev Sergej, Bueso-Ramos Carlos, Medeiros L. Jeffrey, Jorgensen Jeffrey L., Wang Sa A., Flow cytometry immunophenotypic findings in chronic myelomonocytic leukemia and its utility in monitoring treatment response, 10.1111/ejh.12477
  20. Selimoglu-Buet D., Wagner-Ballon O., Saada V., Bardet V., Itzykson R., Bencheikh L., Morabito M., Met E., Debord C., Benayoun E., Nloga A.-M., Fenaux P., Braun T., Willekens C., Quesnel B., Ades L., Fontenay M., Rameau P., Droin N., Koscielny S., Solary E., , Characteristic repartition of monocyte subsets as a diagnostic signature of chronic myelomonocytic leukemia, 10.1182/blood-2015-01-620781
  21. Patnaik Mrinal M., Tefferi Ayalew, Chronic Myelomonocytic Leukemia: Focus on Clinical Practice, 10.1016/j.mayocp.2015.11.011
Bibliographic reference Schillinger, Françoise ; Sourdeau, Elise ; Boubaya, Marouane ; Baseggio, Lucile ; Clauser, Sylvain ; et. al. A new approach for diagnosing chronic myelomonocytic leukemia using structural parameters of Sysmex XNTM analyzers in routine laboratory practice.. In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 78, no. 3, p. 159-164 (2018)
Permanent URL http://hdl.handle.net/2078.1/192606