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Eliminating malignant cells from cryopreserved ovarian tissue is possible in leukaemia patients

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Soares, Michelle [UCL] Saussoy, Pascale [UCL] Maskens, Mathilde [UCL] Reul, Hélène [UCL] Andrade Amorim, Christiani [UCL] Dolmans, Marie-Madeleine [UCL]

(eng) Reimplantation of cryopreserved ovarian tissue (OT) can successfully restore ovarian function in young cancer patients after gonadotoxic treatment. However, for patients with leukaemia, there is a risk of malignant cell transmission. Our objective was to evaluate minimal disseminated disease in OT from leukaemia patients and test a follicle isolation technique to obtain disease-free follicle suspensions. Cryopreserved OT from 12 leukaemia patients was thawed and analysed by histology and long-term xenografting in immunosuppressed mice. In 10 patients, follicles were isolated from OT, and polymerase chain reaction (PCR) was performed on tissue, digested ovarian suspensions and isolated follicle suspensions to investigate leukaemic cell presence. Mean patient age was 17·1 years. An average of 3·2 follicles were isolated per mm² of cortex. Xenografting of OT induced leukaemic masses in 2/12 mice. PCR identified leukaemic cell presence in 66% of OT. Malignant cells were also detected in digested ovarian suspensions. However, none of the follicle samples (>2300 follicles tested) showed any malignant cell presence after washing. This study demonstrates that it is possible to recover large numbers of viable follicles from cryopreserved OT of leukaemia patients. All isolated and washed follicle suspensions tested negative for leukaemic cells, giving leukaemia patients genuine hope of fertility restoration.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2017
Language Anglais
Journal information "British Journal of Haematology" - Vol. 178, no. 2, p. 231-239 (2017)
Peer reviewed yes
Publisher Wiley-Blackwell ((United Kingdom) Oxford)
issn 0007-1048
e-issn 1365-2141
Publication status Publié
Affiliations UCL - SSS/IREC/GYNE - Pôle de Gynécologie
UCL - SSS/IREC/SLUC - Pôle St.-Luc
UCL - (SLuc) Service de biologie hématologique
UCL - (SLuc) Service de gynécologie et d'andrologie
Keywords Oncofertility ; Artificial ovary ; Fertility preservation ; Leukaemia ; Minimal disseminated disease ; Ovarian metastasis
Links
  1. Abir Ronit, Aviram Adina, Feinmesser Meora, Stein Jerry, Yaniv Isaac, Parnes Doris, Ben-Haroush Avi, Meirow Dror, Rabizadeh Esther, Fisch Benjamin, Ovarian minimal residual disease in chronic myeloid leukaemia, 10.1016/j.rbmo.2013.10.011
  2. Amiot C., Angelot-Delettre F., Zver T., Alvergnas-Vieille M., Saas P., Garnache-Ottou F., Roux C., Minimal residual disease detection of leukemic cells in ovarian cortex by eight-color flow cytometry, 10.1093/humrep/det126
  3. Amorim C. A., Van Langendonckt A., David A., Dolmans M.-M., Donnez J., Survival of human pre-antral follicles after cryopreservation of ovarian tissue, follicular isolation and in vitro culture in a calcium alginate matrix, 10.1093/humrep/den343
  4. Bastings L., Beerendonk C. C. M., Westphal J. R., Massuger L. F. A. G., Kaal S. E. J., van Leeuwen F. E., Braat D. D. M., Peek R., Autotransplantation of cryopreserved ovarian tissue in cancer survivors and the risk of reintroducing malignancy: a systematic review, 10.1093/humupd/dmt020
  5. Dolmans M.-M., Safety of ovarian autotransplantation, 10.1182/blood-2012-07-439539
  6. Dolmans Marie-Madeleine, Michaux Nathalie, Camboni Alessandra, Martinez-Madrid Belen, Van Langendonckt Anne, Annarita Nottola Stefania, Donnez Jacques, Evaluation of Liberase, a purified enzyme blend, for the isolation of human primordial and primary ovarian follicles, 10.1093/humrep/dei320
  7. Dolmans M.-M., Marinescu C., Saussoy P., Van Langendonckt A., Amorim C., Donnez J., Reimplantation of cryopreserved ovarian tissue from patients with acute lymphoblastic leukemia is potentially unsafe, 10.1182/blood-2010-01-265751
  8. Dolmans Marie-Madeleine, Luyckx Valérie, Donnez Jacques, Andersen Claus Yding, Greve Tine, Risk of transferring malignant cells with transplanted frozen-thawed ovarian tissue, 10.1016/j.fertnstert.2013.03.027
  9. van Dongen J J M, Langerak A W, Brüggemann M, Evans P A S, Hummel M, Lavender F L, Delabesse E, Davi F, Schuuring E, García-Sanz R, van Krieken J H J M, Droese J, González D, Bastard C, White H E, Spaargaren M, González M, Parreira A, Smith J L, Morgan G J, Kneba M, Macintyre E A, Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936, 10.1038/sj.leu.2403202
  10. Donnez Jacques, Dolmans Marie-Madeleine, Fertility preservation in women, 10.1038/nrendo.2013.205
  11. Donnez Jacques, Dolmans Marie-Madeleine, Ovarian cortex transplantation: 60 reported live births brings the success and worldwide expansion of the technique towards routine clinical practice, 10.1007/s10815-015-0544-9
  12. Donnez Jacques, Silber Sherman, Andersen Claus Yding, Demeestere Isabelle, Piver Pascal, Meirow Dror, Pellicer Antonio, Dolmans Marie-Madeleine, Children born after autotransplantation of cryopreserved ovarian tissue. A review of 13 live births, 10.3109/07853890.2010.546807
  13. Donnez Jacques, Dolmans Marie-Madeleine, Pellicer Antonio, Diaz-Garcia Cesar, Sanchez Serrano Maria, Schmidt Kristen Tryde, Ernst Erik, Luyckx Valérie, Andersen Claus Yding, Restoration of ovarian activity and pregnancy after transplantation of cryopreserved ovarian tissue: a review of 60 cases of reimplantation, 10.1016/j.fertnstert.2013.03.030
  14. Donnez Jacques, Dolmans Marie-Madeleine, Diaz César, Pellicer Antonio, Ovarian cortex transplantation: time to move on from experimental studies to open clinical application, 10.1016/j.fertnstert.2015.08.005
  15. Donnez Jacques, Dolmans Marie-Madeleine, Pellicer Antonio, Diaz-Garcia Cesar, Ernst Erik, Macklon Kirsten T, Andersen Claus Yding, Fertility preservation for age-related fertility decline, 10.1016/s0140-6736(15)60198-2
  16. Edwards Brenda K., Noone Anne-Michelle, Mariotto Angela B., Simard Edgar P., Boscoe Francis P., Henley S. Jane, Jemal Ahmedin, Cho Hyunsoon, Anderson Robert N., Kohler Betsy A., Eheman Christie R., Ward Elizabeth M., Annual Report to the Nation on the status of cancer, 1975-2010, featuring prevalence of comorbidity and impact on survival among persons with lung, colorectal, breast, or prostate cancer : 2013 Report on the Status of Cancer, 10.1002/cncr.28509
  17. Gabert J, Beillard E, van der Velden V H J, Bi W, Grimwade D, Pallisgaard N, Barbany G, Cazzaniga G, Cayuela J M, Cavé H, Pane F, Aerts J L E, De Micheli D, Thirion X, Pradel V, González M, Viehmann S, Malec M, Saglio G, van Dongen J J M, Standardization and quality control studies of ‘real-time’ quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia – A Europe Against Cancer Program, 10.1038/sj.leu.2403135
  18. Greve T., Clasen-Linde E., Andersen M. T., Andersen M. K., Sorensen S. D., Rosendahl M., Ralfkiaer E., Andersen C. Y., Cryopreserved ovarian cortex from patients with leukemia in complete remission contains no apparent viable malignant cells, 10.1182/blood-2012-01-403022
  19. Jahnukainen K, Tinkanen H, Wikström A, Dunkel L, Saarinen-Pihkala U M, Mäkinen S, Asadi Azarbaijani B, Oskam I C, Vettenranta K, Laine T, Kairisto V, Juvonen V, Bone marrow remission status predicts leukemia contamination in ovarian biopsies collected for fertility preservation, 10.1038/leu.2012.279
  20. Luyckx Valérie, Dolmans Marie-Madeleine, Vanacker Julie, Scalercio Sarah R, Donnez Jacques, Amorim Christiani A, First step in developing a 3D biodegradable fibrin scaffold for an artificial ovary, 10.1186/1757-2215-6-83
  21. Luyckx Valérie, Dolmans Marie-Madeleine, Vanacker Julie, Legat Camille, Fortuño Moya Cristina, Donnez Jacques, Amorim Christiani Andrade, A new step toward the artificial ovary: survival and proliferation of isolated murine follicles after autologous transplantation in a fibrin scaffold, 10.1016/j.fertnstert.2013.12.025
  22. Macklon Kirsten T, Jensen Annette Klüver, Loft Anne, Ernst Erik, Andersen Claus Yding, Treatment history and outcome of 24 deliveries worldwide after autotransplantation of cryopreserved ovarian tissue, including two new Danish deliveries years after autotransplantation, 10.1007/s10815-014-0331-z
  23. Martinez-Madrid Belen, Dolmans Marie-Madeleine, Langendonckt Anne Van, Defrère Sylvie, Van Eyck Anne-Sophie, Donnez Jacques, Ficoll density gradient method for recovery of isolated human ovarian primordial follicles, 10.1016/j.fertnstert.2004.05.084
  24. Mayerhofer Klaus, Ott Johannes, Nouri Kazem, Stoegbauer Lucija, Fischer Eva-Maria, Lipovac Markus, Promberger Regina, Huber Johannes C., Laparoscopic ovarian tissue harvesting for cryopreservation: an effective and safe procedure for fertility preservation, 10.1016/j.ejogrb.2010.05.034
  25. Meirow D., Hardan I., Dor J., Fridman E., Elizur S., Ra'anani H., Slyusarevsky E., Amariglio N., Schiff E., Rechavi G., Nagler A., Yehuda D. B., Searching for evidence of disease and malignant cell contamination in ovarian tissue stored from hematologic cancer patients, 10.1093/humrep/den055
  26. Ovarian tissue cryopreservation: a committee opinion, 10.1016/j.fertnstert.2014.02.052
  27. Rosendahl Mikkel, Andersen Morten Tolstrup, Ralfkiær Elisabeth, Kjeldsen Lars, Andersen Mette Klarskov, Andersen Claus Yding, Evidence of residual disease in cryopreserved ovarian cortex from female patients with leukemia, 10.1016/j.fertnstert.2009.11.032
  28. Rosendahl Mikkel, Schmidt Kirsten Tryde, Ernst Erik, Rasmussen Per Emil, Loft Anne, Byskov Anne Grethe, Andersen Anders Nyboe, Andersen Claus Yding, Cryopreservation of ovarian tissue for a decade in Denmark: a view of the technique, 10.1016/j.rbmo.2010.10.015
  29. Rosendahl Mikkel, Greve Tine, Andersen Claus Yding, The safety of transplanting cryopreserved ovarian tissue in cancer patients: a review of the literature, 10.1007/s10815-012-9912-x
  30. Sánchez María, Novella-Maestre Edurne, Teruel José, Ortiz Ester, Pellicer Antonio, Mirabet V., The valencia programme for fertility preservation, 10.1007/s12094-008-0227-4
  31. Smitz J., Dolmans M. M., Donnez J., Fortune J. E., Hovatta O., Jewgenow K., Picton H. M., Plancha C., Shea L. D., Stouffer R. L., Telfer E. E., Woodruff T. K., Zelinski M. B., Current achievements and future research directions in ovarian tissue culture, in vitro follicle development and transplantation: implications for fertility preservation, 10.1093/humupd/dmp056
  32. Soares Michelle, Sahrari Karima, Amorim Christiani Andrade, Saussoy Pascale, Donnez Jacques, Dolmans Marie-Madeleine, Evaluation of a human ovarian follicle isolation technique to obtain disease-free follicle suspensions before safely grafting to cancer patients, 10.1016/j.fertnstert.2015.05.021
  33. Telfer Evelyn, McLaughlin Marie, In Vitro Development of Ovarian Follicles, 10.1055/s-0030-1268700
  34. Telfer Evelyn E., Zelinski Mary B., Ovarian follicle culture: advances and challenges for human and nonhuman primates, 10.1016/j.fertnstert.2013.03.043
  35. Vanacker Julie, Camboni Alessandra, Dath Catherine, Van Langendonckt Anne, Dolmans Marie-Madeleine, Donnez Jacques, Amorim Christiani A., Enzymatic isolation of human primordial and primary ovarian follicles with Liberase DH: protocol for application in a clinical setting, 10.1016/j.fertnstert.2011.05.075
  36. Vanacker Julie, Dolmans Marie-Madeleine, Luyckx Valérie, Donnez Jacques, Amorim Christiani A, First transplantation of isolated murine follicles in alginate, 10.2217/rme.14.33
  37. Ward, CA: A Cancer Journal for Clinicians, 64, 83 (2014)
  38. Zver T., Alvergnas-Vieille M., Garnache-Ottou F., Ferrand C., Roux C., Amiot C., Minimal residual disease detection in cryopreserved ovarian tissue by multicolor flow cytometry in acute myeloid leukemia, 10.3324/haematol.2014.113373
  39. Zver, Journal of Assisted Reproduction and Genetics, 29, 1059 (2015)
Bibliographic reference Soares, Michelle ; Saussoy, Pascale ; Maskens, Mathilde ; Reul, Hélène ; Andrade Amorim, Christiani ; et. al. Eliminating malignant cells from cryopreserved ovarian tissue is possible in leukaemia patients. In: British Journal of Haematology, Vol. 178, no. 2, p. 231-239 (2017)
Permanent URL http://hdl.handle.net/2078.1/188516