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Novel scanning procedure enabling the vectorization of entire rhizotron-grown root systems.

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Lobet, Guillaume [UCL] Draye, Xavier [UCL]

: This paper presents an original spit-and-combine imaging procedure that enables the complete vectorization of complex root systems grown in rhizotrons. The general principle of the method is to (1) separate the root system into a small number of large pieces to reduce root overlap, (2) scan these pieces one by one, (3) analyze separate images with a root tracing software and (4) combine all tracings into a single vectorized root system. This method generates a rich dataset containing morphological, topological and geometrical information of entire root systems grown in rhizotrons. The utility of the method is illustrated with a detailed architectural analysis of a 20-day old maize root system, coupled with a spatial analysis of water uptake patterns.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2013
Language Anglais
Journal information "Plant Methods" - Vol. 9, no.1, p. 1 (2013)
Peer reviewed yes
Publisher BioMed Central Ltd. (London)
issn 1746-4811
e-issn 1746-4811
Publication status Publié
Affiliation UCL - SST/ELI/ELIA - Agronomy
Links
  1. Ge Zhenyang, Rubio Gerardo, Lynch Jonathan P, 10.1023/a:1014987710937
  2. Draye Xavier, Kim Yangmin, Lobet Guillaume, Javaux Mathieu, Model-assisted integration of physiological and environmental constraints affecting the dynamic and spatial patterns of root water uptake from soils, 10.1093/jxb/erq077
  3. Downie Helen, Holden Nicola, Otten Wilfred, Spiers Andrew J., Valentine Tracy A., Dupuy Lionel X., Transparent Soil for Imaging the Rhizosphere, 10.1371/journal.pone.0044276
  4. Lobet Guillaume, Pagès Loïc, Draye Xavier, A Novel Image-Analysis Toolbox Enabling Quantitative Analysis of Root System Architecture, 10.1104/pp.111.179895
  5. Barthélémy Daniel, Caraglio Yves, Plant Architecture: A Dynamic, Multilevel and Comprehensive Approach to Plant Form, Structure and Ontogeny, 10.1093/aob/mcl260
  6. Galkovskyi Taras, Mileyko Yuriy, Bucksch Alexander, Moore Brad, Symonova Olga, Price Charles A, Topp Christopher N, Iyer-Pascuzzi Anjali S, Zurek Paul R, Fang Suqin, Harer John, Benfey Philip N, Weitz Joshua S, GiA Roots: software for the high throughput analysis of plant root system architecture, 10.1186/1471-2229-12-116
  7. Postma J. A., Lynch J. P., Root Cortical Aerenchyma Enhances the Growth of Maize on Soils with Suboptimal Availability of Nitrogen, Phosphorus, and Potassium, 10.1104/pp.111.175489
  8. Javot H., Role of a Single Aquaporin Isoform in Root Water Uptake, 10.1105/tpc.008888
Bibliographic reference Lobet, Guillaume ; Draye, Xavier. Novel scanning procedure enabling the vectorization of entire rhizotron-grown root systems.. In: Plant Methods, Vol. 9, no.1, p. 1 (2013)
Permanent URL http://hdl.handle.net/2078.1/186201