User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Identification of a naturally processed HLA-A*02:01-restricted CTL epitope from the human tumor-associated antigen Nectin-4.

Primary tabs

Lopez, Marc Ghidouche, Abderrezak Rochas, Caroline Godelaine, Danièle Carrasco, Javier Colau, Didier [UCL] Hames, Gérald [UCL] Coulie, Pierre G. [UCL] Olive, Daniel

Nectin-4 is a tumor antigen present on the surface of breast, ovarian and lung carcinoma cells. It is rarely present in normal adult tissues and is therefore a candidate target for cancer immunotherapy. Here, we identified a Nectin-4 antigenic peptide that is naturally presented to T cells by HLA-A2 molecules. We first screened the 502 nonamer peptides of Nectin-4 (510 amino acids) for binding to and off-rate from eight different HLA class I molecules. We then combined biochemical, cellular and algorithmic assays to select 5 Nectin-4 peptides that bound to HLA-A*02:01 molecules. Cytolytic T lymphocytes were obtained from healthy donors, that specifically lyzed HLA-A2(+) cells pulsed with 2 out of the 5 peptides, indicating the presence of anti-Nectin-4 CD8(+) T lymphocytes in the human T cell repertoire. Finally, an HLA-A2-restricted cytolytic T cell clone derived from a breast cancer patient recognized peptide Nectin-4145-153 (VLVPPLPSL) and lyzed HLA-A2(+) Nectin-4(+) breast carcinoma cells. These results indicate that peptide Nectin-4145-153 is naturally processed for recognition by T cells on HLA-A2 molecules. It could be used to monitor antitumor T cell responses or to immunize breast cancer patients.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2016
Language Anglais
Journal information "Cancer Immunology, Immunotherapy : other biological response modifications" - Vol. 65, no.10, p. 1177-1188 (2016)
Peer reviewed yes
Publisher Springer (Heidelberg)
issn 0340-7004
e-issn 1432-0851
Publication status Publié
Affiliation UCL - SSS/DDUV - Institut de Duve
Links
  1. Sliwkowski M. X., Mellman I., Antibody Therapeutics in Cancer, 10.1126/science.1241145
  2. Coulie Pierre G., Van den Eynde Benoît J., van der Bruggen Pierre, Boon Thierry, Tumour antigens recognized by T lymphocytes: at the core of cancer immunotherapy, 10.1038/nrc3670
  3. Fabre-Lafay Stéphanie, Monville Florence, Garrido-Urbani Sarah, Berruyer-Pouyet Carole, Ginestier Christophe, Reymond Nicolas, Finetti Pascal, Sauvan Richard, Adélaïde José, Geneix Jeannine, Lecocq Eric, Popovici Cornel, Dubreuil Patrice, Viens Patrice, Gonçalves Anthony, Charafe-Jauffret Emmanuelle, Jacquemier Jocelyne, Birnbaum Daniel, Lopez Marc, Nectin-4 is a new histological and serological tumor associated marker for breast cancer, 10.1186/1471-2407-7-73
  4. Reymond Nicolas, Fabre Stéphanie, Lecocq Eric, Adelaı̈de José, Dubreuil Patrice, Lopez Marc, Nectin4/PRR4, a New Afadin-associated Member of the Nectin Family That Trans-interacts with Nectin1/PRR1 through V Domain Interaction, 10.1074/jbc.m103810200
  5. Fabre-Lafay Stéphanie, Garrido-Urbani Sarah, Reymond Nicolas, Gonçalves Anthony, Dubreuil Patrice, Lopez Marc, Nectin-4, a New Serological Breast Cancer Marker, Is a Substrate for Tumor Necrosis Factor-α-converting Enzyme (TACE)/ADAM-17, 10.1074/jbc.m410943200
  6. Takai Yoshimi, Ikeda Wataru, Ogita Hisakazu, Rikitake Yoshiyuki, The Immunoglobulin-Like Cell Adhesion Molecule Nectin and Its Associated Protein Afadin, 10.1146/annurev.cellbio.24.110707.175339
  7. Takai Y., Nectin and afadin: novel organizers of intercellular junctions, 10.1242/jcs.00167
  8. Reymond Nicolas, Imbert Anne-Marie, Devilard Elisabeth, Fabre Stéphanie, Chabannon Christian, Xerri Luc, Farnarier Catherine, Cantoni Claudia, Bottino Cristina, Moretta Alessandro, Dubreuil Patrice, Lopez Marc, DNAM-1 and PVR Regulate Monocyte Migration through Endothelial Junctions, 10.1084/jem.20032206
  9. Takai Yoshimi, Miyoshi Jun, Ikeda Wataru, Ogita Hisakazu, Nectins and nectin-like molecules: roles in contact inhibition of cell movement and proliferation, 10.1038/nrm2457
  10. Pende Daniela, Bottino Cristina, Castriconi Roberta, Cantoni Claudia, Marcenaro Stefania, Rivera Paola, Spaggiari Grazia M., Dondero Alessandra, Carnemolla Barbara, Reymond Nicolas, Mingari Maria Cristina, Lopez Marc, Moretta Lorenzo, Moretta Alessandro, PVR (CD155) and Nectin-2 (CD112) as ligands of the human DNAM-1 (CD226) activating receptor: involvement in tumor cell lysis, 10.1016/j.molimm.2004.07.028
  11. Kuramochi Masami, Fukuhara Hiroshi, Nobukuni Takahiro, Kanbe Takamasa, Maruyama Tomoko, Ghosh Hara P., Pletcher Mathew, Isomura Minoru, Onizuka Masataka, Kitamura Tadaichi, Sekiya Takao, Reeves Roger H., Murakami Yoshinori, 10.1038/86934
  12. Brancati Francesco, Fortugno Paola, Bottillo Irene, Lopez Marc, Josselin Emmanuelle, Boudghene-Stambouli Omar, Agolini Emanuele, Bernardini Laura, Bellacchio Emanuele, Iannicelli Miriam, Rossi Alfredo, Dib-Lachachi Amina, Stuppia Liborio, Palka Giandomenico, Mundlos Stefan, Stricker Sigmar, Kornak Uwe, Zambruno Giovanna, Dallapiccola Bruno, Mutations in PVRL4, Encoding Cell Adhesion Molecule Nectin-4, Cause Ectodermal Dysplasia-Syndactyly Syndrome, 10.1016/j.ajhg.2010.07.003
  13. Muhlebach MD, Mateo M, Sinn PL, Prufer S, Uhlig KM, Leonard VH, Navaratnarajah CK, Frenzke M, Wong XX, Sawatsky B, Ramachandran S, McCray PB Jr, Cichutek K, von Messling V, Lopez M, Cattaneo R (2011) Adherens junction protein nectin-4 is the epithelial receptor for measles virus. Nature 480(7378):530–533
  14. Campadelli-Fiume Gabriella, Cocchi Francesca, Menotti Laura, Lopez Marc, The novel receptors that mediate the entry of herpes simplex viruses and animal alphaherpesviruses into cells, 10.1002/1099-1654(200009/10)10:5<305::aid-rmv286>3.0.co;2-t
  15. DeRycke Melissa S., Pambuccian Stefan E., Gilks C. Blake, Kalloger Steve E., Ghidouche Abderrezak, Lopez Marc, Bliss Robin L., Geller Melissa A., Argenta Peter A., Harrington Katherine M., Skubitz Amy P.N., Nectin 4 Overexpression in Ovarian Cancer Tissues and Serum : Potential Role as a Serum Biomarker, 10.1309/ajcpgxk0fr4mhihb
  16. Takano A., Ishikawa N., Nishino R., Masuda K., Yasui W., Inai K., Nishimura H., Ito H., Nakayama H., Miyagi Y., Tsuchiya E., Kohno N., Nakamura Y., Daigo Y., Identification of Nectin-4 Oncoprotein as a Diagnostic and Therapeutic Target for Lung Cancer, 10.1158/0008-5472.can-09-0016
  17. Fabre Stéphanie, Reymond Nicolas, Cocchi Francesca, Menotti Laura, Dubreuil Patrice, Campadelli-Fiume Gabriella, Lopez Marc, Prominent Role of the Ig-like V Domain intrans-Interactions of Nectins : NECTIN3 AND NECTIN4 BIND TO THE PREDICTED C-C′-C"-D β-STRANDS OF THE NECTIN1 V DOMAIN, 10.1074/jbc.m203228200
  18. Guilloux Y., A peptide recognized by human cytolytic T lymphocytes on HLA-A2 melanomas is encoded by an intron sequence of the N- acetylglucosaminyltransferase V gene, 10.1084/jem.183.3.1173
  19. Kawashima Ichiro, Hudson Stephen J, Tsai Van, Southwood Scott, Takesako Kazutoh, Appella Ettore, Sette Alessandro, Celis Esteban, The Multi-epitope Approach for Immunotherapy for Cancer: Identification of Several CTL Epitopes from Various Tumor-Associated Antigens Expressed on Solid Epithelial Tumors, 10.1016/s0198-8859(97)00255-3
  20. Huang L-Q, Brasseur F, Serrano A, De Plaen E, van der Bruggen P, Boon T, Van Pel A (1999) Cytolytic T lymphocytes recognize an antigen encoded by MAGE-A10 on a human melanoma. J Immunol 162:6849–6854
  21. Ma Wenbin, Germeau Catherine, Vigneron Nathalie, Maernoudt Anne-Sophie, Morel Sandra, Boon Thierry, Coulie Pierre G., Van den Eynde Beno�t J., Two new tumor-specific antigenic peptides encoded by geneMAGE-C2 and presented to cytolytic T lymphocytes by HLA-A2, 10.1002/ijc.20038
  22. Ma Wenbin, Vigneron Nathalie, Chapiro Jacques, Stroobant Vincent, Germeau Catherine, Boon Thierry, Coulie Pierre G., Van den Eynde Benoît J., A MAGE-C2 antigenic peptide processed by the immunoproteasome is recognized by cytolytic T cells isolated from a melanoma patient after successful immunotherapy, 10.1002/ijc.25911
  23. Rimoldi D., Rubio-Godoy V., Dutoit V., Lienard D., Salvi S., Guillaume P., Speiser D., Stockert E., Spagnoli G., Servis C., Cerottini J.-C., Lejeune F., Romero P., Valmori D., Efficient Simultaneous Presentation of NY-ESO-1/LAGE-1 Primary and Nonprimary Open Reading Frame-Derived CTL Epitopes in Melanoma, 10.4049/jimmunol.165.12.7253
  24. Ikeda Hideyuki, Lethé Bernard, Lehmann Frédéric, Van Baren Nicolas, Baurain Jean-François, De Smet Charles, Chambost Hervé, Vitale Massimo, Moretta Alessandro, Boon Thierry, Coulie Pierre G, Characterization of an Antigen That Is Recognized on a Melanoma Showing Partial HLA Loss by CTL Expressing an NK Inhibitory Receptor, 10.1016/s1074-7613(00)80426-4
  25. Molldrem JJ, Clave E, Jiang YZ, Mavroudis D, Raptis A, Hensel N, Agarwala V, Barrett AJ (1997) Cytotoxic T lymphocytes specific for a nonpolymorphic proteinase 3 peptide preferentially inhibit chronic myeloid leukemia colony-forming units. Blood 90(7):2529–2534
  26. Wölfel Thomas, Van Pel Aline, Brichard Vincent, Schneider Jörg, Seliger Barbara, Zum Büschenfelde Karl-Hermann Meyer, Boon Thierry, Two tyrosinase nonapeptides recognized on HLA-A2 melanomas by autologous cytolytic T lymphocytes, 10.1002/eji.1830240340
  27. Doytchinova I. A., Walshe V. A., Jones N. A., Gloster S. E., Borrow P., Flower D. R., Coupling In Silico and In Vitro Analysis of Peptide-MHC Binding: A Bioinformatic Approach Enabling Prediction of Superbinding Peptides and Anchorless Epitopes, 10.4049/jimmunol.172.12.7495
  28. Nijman Hans W., Houbiers Jos G. A., Vierboom Michel P. M., van der Burg Sjoerd H., Drijfhout Jan W., D'Amaro Joe, Kenemans Peter, Melief Cornelis J. M., Kast W. Martin, Identification of peptide sequences that potentially trigger HLA-A2.1-restricted cytotoxic T lymphocytes, 10.1002/eji.1830230603
  29. Serody J. S., Collins E. J., Tisch R. M., Kuhns J. J., Frelinger J. A., T Cell Activity After Dendritic Cell Vaccination Is Dependent on Both the Type of Antigen and the Mode of Delivery, 10.4049/jimmunol.164.9.4961
  30. Strous GJ, van Kerkhof P, van Meer G, Rijnboutt S, Stoorvogel W (1993) Differential effects of brefeldin A on transport of secretory and lysosomal proteins. J Biol Chem 268(4):2341–2347
  31. DiBrino M, Tsuchida T, Turner RV, Parker KC, Coligan JE, Biddison WE (1993) HLA-A1 and HLA-A3 T cell epitopes derived from influenza virus proteins predicted from peptide binding motifs. J Immunol 151(11):5930–5935
  32. Baurain J.-F., Colau D., van Baren N., Landry C., Martelange V., Vikkula M., Boon T., Coulie P. G., High Frequency of Autologous Anti-Melanoma CTL Directed Against an Antigen Generated by a Point Mutation in a New Helicase Gene, 10.4049/jimmunol.164.11.6057
  33. Garboczi D. N., Hung D. T., Wiley D. C., HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides., 10.1073/pnas.89.8.3429
  34. Boccaccio Claire, Jacod Sylvie, Kaiser Andrew, Boyer Aurélie, Abastado Jean-Pierre, Nardin Alessandra, Identification of a Clinical-Grade Maturation Factor for Dendritic Cells : , 10.1097/00002371-200201000-00010
  35. Ottaviani Sabrina, Zhang Yi, Boon Thierry, van der Bruggen Pierre, A MAGE-1 antigenic peptide recognized by human cytolytic T lymphocytes on HLA-A2 tumor cells, 10.1007/s00262-005-0705-2
  36. Karanikas V., Lurquin C., Colau D., van Baren N., De Smet C., Lethe B., Connerotte T., Corbiere V., Demoitie M.-A., Lienard D., Dreno B., Velu T., Boon T., Coulie P. G., Monoclonal Anti-MAGE-3 CTL Responses in Melanoma Patients Displaying Tumor Regression after Vaccination with a Recombinant Canarypox Virus, 10.4049/jimmunol.171.9.4898
  37. Godelaine D., Carrasco J., Lucas S., Karanikas V., Schuler-Thurner B., Coulie P. G., Schuler G., Boon T., Van Pel A., Polyclonal CTL Responses Observed in Melanoma Patients Vaccinated with Dendritic Cells Pulsed with a MAGE-3.A1 Peptide, 10.4049/jimmunol.171.9.4893
  38. Bachinsky MM, Guillen DE, Patel SR, Singleton J, Chen C, Soltis DA, Tussey LG (2005) Mapping and binding analysis of peptides derived from the tumor-associated antigen survivin for eight HLA alleles. Cancer Immun 5:6
  39. Shingler W. H., Chikoti P., Kingsman S. M., Harrop R., Identification and functional validation of MHC class I epitopes in the tumor-associated antigen 5T4, 10.1093/intimm/dxn063
  40. Thome Joseph J.C., Yudanin Naomi, Ohmura Yoshiaki, Kubota Masaru, Grinshpun Boris, Sathaliyawala Taheri, Kato Tomoaki, Lerner Harvey, Shen Yufeng, Farber Donna L., Spatial Map of Human T Cell Compartmentalization and Maintenance over Decades of Life, 10.1016/j.cell.2014.10.026
  41. Parker KC, Bednarek MA, Coligan JE (1994) Scheme for ranking potential HLA-A2 binding peptides based on independent binding of individual peptide side-chains. J Immunol 152(1):163–175
  42. Vigneron N, Stroobant V, Van den Eynde BJ, van der Bruggen P (2013) Database of T cell-defined human tumor antigens: the 2013 update. Cancer Immun 13:15
Bibliographic reference Lopez, Marc ; Ghidouche, Abderrezak ; Rochas, Caroline ; Godelaine, Danièle ; Carrasco, Javier ; et. al. Identification of a naturally processed HLA-A*02:01-restricted CTL epitope from the human tumor-associated antigen Nectin-4.. In: Cancer Immunology, Immunotherapy : other biological response modifications, Vol. 65, no.10, p. 1177-1188 (2016)
Permanent URL http://hdl.handle.net/2078.1/182271