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Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.

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Renard, Henri-François [UCL] Simunovic, Mijo Lemière, Joël Boucrot, Emmanuel Garcia-Castillo, Maria Daniela Johannes, Ludger

During endocytosis, energy is invested to narrow the necks of cargo-containing plasma membrane invaginations to radii at which the opposing segments spontaneously coalesce, thereby leading to the detachment by scission of endocytic uptake carriers. In the clathrin pathway, dynamin uses mechanical energy from GTP hydrolysis to this effect, assisted by the BIN/amphiphysin/Rvs (BAR) domain-containing protein endophilin. Clathrin-independent endocytic events are often less reliant on dynamin, and whether in these cases BAR domain proteins such as endophilin contribute to scission has remained unexplored. Here we show, in human and other mammalian cell lines, that endophilin-A2 (endoA2) specifically and functionally associates with very early uptake structures that are induced by the bacterial Shiga and cholera toxins, which are both clathrin-independent endocytic cargoes. In controlled in vitro systems, endoA2 reshapes membranes before scission. Furthermore, we demonstrate that endoA2, dynamin and actin contribute in parallel to the scission of Shiga-toxin-induced tubules. Our results establish a novel function of endoA2 in clathrin-independent endocytosis. They document that distinct scission factors operate in an additive manner, and predict that specificity within a given uptake process arises from defined combinations of universal modules. Our findings highlight a previously unnoticed link between membrane scaffolding by endoA2 and pulling-force-driven dynamic scission.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2015
Language Anglais
Journal information "Nature" - Vol. 517, no. 7535, p. 493-496 (2014)
Peer reviewed yes
Publisher Nature Publishing Group (London)
issn 0028-0836
e-issn 1476-4687
Publication status Publié
Affiliations UCL - SST/ISV - Institut des sciences de la vie
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
MESH Subject Actins ; Humans ; Rats ; Shiga Toxin ; Acyltransferases ; Animals ; Cell Line ; Cell Membrane ; Cholera Toxin ; Clathrin ; Dynamins ; Endocytosis
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Bibliographic reference Renard, Henri-François ; Simunovic, Mijo ; Lemière, Joël ; Boucrot, Emmanuel ; Garcia-Castillo, Maria Daniela ; et. al. Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.. In: Nature, Vol. 517, no. 7535, p. 493-496 (2014)
Permanent URL http://hdl.handle.net/2078.1/180305