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Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport.

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Rydell, Gustaf E Renard, Henri-François [UCL] Garcia-Castillo, Maria-Daniela Dingli, Florent Loew, Damarys Johannes, Ludger

Several exogenous and endogenous cargo proteins are internalized independently of clathrin, including the bacterial Shiga toxin. The mechanisms underlying early steps of clathrin-independent uptake remain largely unknown. In this study, we have designed a protocol to obtain gradient fractions containing Shiga toxin internalization intermediates. Using stable isotope labeling with amino acids in cell culture (SILAC) and quantitative mass spectrometry, Rab12 was found in association with these very early uptake carriers. The localization of the GTPase on Shiga toxin-induced plasma membrane invaginations was shown by fluorescence microscopy in cells transfected with GFP-Rab12. Furthermore, using a quantitative biochemical assay, it was found that the amount of receptor-binding B-subunit of Shiga toxin reaching the trans-Golgi/TGN membranes was decreased in Rab12-depleted cells, and that cells were partially protected against intoxication by Shiga-like toxin 1 under these conditions. These findings demonstrate the functional importance of Rab12 for retrograde toxin trafficking. Among several other intracellular transport pathways, only the steady-state localizations of TGN46 and cation-independent mannose-6-phosphate receptor were affected. These data thus strongly suggest that Rab12 functions in the retrograde transport route.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2014
Language Anglais
Journal information "Traffic" - Vol. 15, no. 7, p. 772-787 (2014)
Peer reviewed yes
Publisher Wiley-Blackwell Publishing, Inc. ((United States) Malden)
issn 1398-9219
e-issn 1600-0854
Publication status Publié
Affiliations UCL - SST/ISV - Institut des sciences de la vie
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
MESH Subject Cell Membrane ; Endocytosis ; HeLa Cells ; Humans ; Protein Transport ; Shiga Toxin ; rab GTP-Binding Proteins
Links
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Bibliographic reference Rydell, Gustaf E ; Renard, Henri-François ; Garcia-Castillo, Maria-Daniela ; Dingli, Florent ; Loew, Damarys ; et. al. Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport.. In: Traffic, Vol. 15, no. 7, p. 772-787 (2014)
Permanent URL http://hdl.handle.net/2078.1/180288