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Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes.

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Romanov, Roman A Zeisel, Amit Bakker, Joanne Girach, Fatima Hellysaz, Arash Clotman, Frédéric [UCL] Harkany, Tibor

The hypothalamus contains the highest diversity of neurons in the brain. Many of these neurons can co-release neurotransmitters and neuropeptides in a use-dependent manner. Investigators have hitherto relied on candidate protein-based tools to correlate behavioral, endocrine and gender traits with hypothalamic neuron identity. Here we map neuronal identities in the hypothalamus by single-cell RNA sequencing. We distinguished 62 neuronal subtypes producing glutamatergic, dopaminergic or GABAergic markers for synaptic neurotransmission and harboring the ability to engage in task-dependent neurotransmitter switching. We identified dopamine neurons that uniquely coexpress the Onecut3 and Nmur2 genes, and placed these in the periventricular nucleus with many synaptic afferents arising from neuromedin S(+) neurons of the suprachiasmatic nucleus. These neuroendocrine dopamine cells may contribute to the dopaminergic inhibition of prolactin secretion diurnally, as their neuromedin S(+) inputs originate from neurons expressing Per2 and Per3 and their tyrosine hydroxylase phosphorylation is regulated in a circadian fashion. Overall, our catalog of neuronal subclasses provides new understanding of hypothalamic organization and function.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2016
Language Anglais
Journal information "Nature Neuroscience" - Vol. 20, no. 2, p. 176-188 (Feb 2017)
Peer reviewed yes
Publisher Nature Publishing Group (New York)
issn 1097-6256
e-issn 1546-1726
Publication status Publié
Affiliation UCL - SSS/IONS/CEMO - Pôle Cellulaire et moléculaire
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Bibliographic reference Romanov, Roman A ; Zeisel, Amit ; Bakker, Joanne ; Girach, Fatima ; Hellysaz, Arash ; et. al. Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes.. In: Nature Neuroscience, Vol. 20, no. 2, p. 176-188 (Feb 2017)
Permanent URL http://hdl.handle.net/2078.1/179914