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Effects of tail suspension on serum testosterone and molecular targets regulating muscle mass

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De Naeyer, Hélène Lamon, Séverine Russell, Aaron P Everaert, Inge De Spaey, Annelies Jamart, Cécile [UCL] Derave, Wim

Introduction: The contribution of reduced testosterone levels to tail suspension (TS)-induced muscle atrophy remains equivocal. The molecular mechanism by which testosterone regulates muscle mass during TS has not been investigated. Methods: Effects of TS on serum testosterone levels, muscle mass, and expression of muscle atrophy- and hypertrophy-inducing targets were measured in soleus (SOL) and extensor digitorum longus (EDL) muscles after testosterone administration during 1, 5, and 14 days of TS in male mice. Results: TS produced an increase followed by a transient drop in testosterone levels. Muscle atrophy was associated with downregulation of Igf1 and upregulation of Mstn, Redd1, Atrogin-1, and MuRF1 mRNA with clear differences in Igf1, Mstn, and MAFbx/Atrogin-1 gene expression between SOL and EDL. Testosterone supplementation did not affect muscle mass or protein expression levels during TS. Conclusions The known anabolic effects of testosterone are not sufficient to ameliorate loss of muscle mass during TS

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2015
Language Anglais
Journal information "Muscle & Nerve" - Vol. 52, no.2, p. 278-288 (2015)
Peer reviewed yes
Publisher JohnWiley & Sons, Inc. ((United States) Hoboken, NJ)
issn 0148-639X
e-issn 1097-4598
Publication status Publié
Affiliation UCL - SSS/IONS/CEMO - Pôle Cellulaire et moléculaire
MESH Subject Animals ; Random Allocation ; Testosterone ; Biomarkers ; Gene Expression Regulation ; Hindlimb Suspension ; Male ; Mice ; Mice, Inbred C57BL ; Muscular Atrophy ; RNA
Keywords AKT ; Atrogin-1 ; Disuse atrophy ; Tail suspension ; Testosterone
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Bibliographic reference De Naeyer, Hélène ; Lamon, Séverine ; Russell, Aaron P ; Everaert, Inge ; De Spaey, Annelies ; et. al. Effects of tail suspension on serum testosterone and molecular targets regulating muscle mass. In: Muscle & Nerve, Vol. 52, no.2, p. 278-288 (2015)
Permanent URL http://hdl.handle.net/2078.1/175833