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Dual stimuli-responsive coating designed through layer-by-layer assembly of PAA-b-PNIPAM block copolymers for the control of protein adsorption

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Osypova, Alina [UCL] Magnin, Delphine [UCL] Sibret, P. Aqil, A. Jerôme, Christine [Université de Liège] Dupont-Gillain, Christine C. [UCL] Demoustier-Champagne, Sophie [UCL] Landoulsi, J.

In this paper, we describe the successful construction, characteristics and interaction with proteins of stimuli-responsive thin nanostructured films prepared by layer-by-layer (LbL) sequential assembly of PNIPAM-containing polyelectrolytes and PAH. PAA-b-PNIPAM block copolymers were synthesized in order to benefit from (i) the ionizable properties of PAA, to be involved in the LbL assembly, and (ii) the sensitivity of PNIPAM to temperature stimulus. The impact of parameters related to the structure and size of the macromolecules (their molecular weight and the relative degree of polymerization of PAA and PNIPAM), and the interaction with proteins under physico-chemical stimuli, such as pH and temperature, are carefully investigated. The incorporation of PAA-b-PNIPAM into multilayered films is shown to be successful whatever the block copolymer used, resulting in slightly thicker films than the corresponding (PAA/PAH)n film. Importantly, the protein adsorption studies demonstrate that it is possible to alter the adsorption behavior of proteins on (PAA-b-PNIPAM/PAH)n surfaces by varying the temperature and/or the pH of the medium, which seems to be intimately related to two key factors: (i) the ability of PNIPAM units to undergo conformational changes and (ii) the structural changes of the film made of weak polyelectrolytes. The simplicity of construction of these PNIPAM block copolymer-based LbL coatings on a large range of substrates, combined with their highly tunable features, make them ideal candidates to be employed for various biomedical applications requiring the control of protein adsorption

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2015
Language Anglais
Journal information "Soft Matter" - Vol. 11, no.41, p. 8154-8164 (2015)
Peer reviewed yes
Publisher Royal Society of Chemistry
issn 1744-683X
e-issn 1744-6848
Publication status Publié
Affiliation UCL - SST/IMCN/BSMA - Bio and soft matter
Links
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Bibliographic reference Osypova, Alina ; Magnin, Delphine ; Sibret, P. ; Aqil, A. ; Jerôme, Christine ; et. al. Dual stimuli-responsive coating designed through layer-by-layer assembly of PAA-b-PNIPAM block copolymers for the control of protein adsorption. In: Soft Matter, Vol. 11, no.41, p. 8154-8164 (2015)
Permanent URL http://hdl.handle.net/2078.1/171245