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Characterization of vegetative inflorescence (mc-vin) mutant provides new insight into the role of MACROCALYX in regulating inflorescence development of tomato.

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Yuste-Lisbona, Fernando [Centro de investigacion en biotechnologica agroalimentaria, Universidad de Almeria] Quinet, Muriel [UCL] Fernandez-Lozano, Antonia [Centro de investigacion en biotechnologica agroalimentaria, Universidad de Almeria] Pineda, Benito [Instituto de biologica molecular y celular de plantas, Universidad politecnica de Valencia] Moreno, Vincente [Instituto de biologica molecular y celular de plantas, Universidad politecnica de Valencia] Lozano, Rafael [Centro de investigacion en biotechnologica agroalimentaria, Universidad de Almeria]

Inflorescence development is a key factor of plant productivity, as it determines flower number. Therefore, understanding the mechanisms that regulate inflorescence architecture is critical for reproductive success and crop yield. In this study, a new mutant, vegetative inflorescence (mc-vin), was isolated from the screening of a tomato (Solanum lycopersicum L.) T-DNA mutant collection. The mc-vin mutant developed inflorescences that reverted to vegetative growth after forming two to three flowers, indicating that the mutated gene is essential for the maintenance of inflorescence meristem identity. The T-DNA was inserted into the promoter region of the MACROCALYX (MC) gene; this result together with complementation test and expression analyses proved that mc-vin is a new knock-out allele of MC. Double combinations between mc-vin and jointless (j) and single flower truss (sft) inflorescence mutants showed that MC has pleiotropic effects on the reproductive phase, and that it interacts with SFT and J to control floral transition and inflorescence fate in tomato. In addition, MC expression was mis-regulated in j and sft mutants whereas J and SFT were significantly up-regulated in the mc-vin mutant. Together, these results provide new evidences about MC function as part of the genetic network regulating the development of tomato inflorescence meristem.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès libre
Publication date 2016
Language Anglais
Journal information "Scientific Reports" - Vol. 6, p. 18796 (2016)
Peer reviewed yes
issn 2045-2322
e-issn 2045-2322
Publication status Publié
Affiliation UCL - SST/ELI/ELIA - Agronomy
Links
  1. Benlloch R., Berbel A., Serrano-Mislata A., Madueno F., Floral Initiation and Inflorescence Architecture: A Comparative View, 10.1093/aob/mcm146
  2. Andrés Fernando, Coupland George, The genetic basis of flowering responses to seasonal cues, 10.1038/nrg3291
  3. Song Young Hun, Ito Shogo, Imaizumi Takato, Flowering time regulation: photoperiod- and temperature-sensing in leaves, 10.1016/j.tplants.2013.05.003
  4. Ó'Maoiléidigh Diarmuid Seosamh, Graciet Emmanuelle, Wellmer Frank, Gene networks controllingArabidopsis thalianaflower development, 10.1111/nph.12444
  5. Quinet, M. & Kinet, J. M. Transition to flowering and morphogenesis of reproductive structures in tomato. Int. J. Dev. Biol. 1, 64–74 (2007).
  6. Samach Alon, Lotan Hamutal, The transition to flowering in tomato, 10.5511/plantbiotechnology.24.71
  7. Lozano Rafael, Gimenez Estela, Cara Beatriz, Capel Juan, Angosto Trinidad, Genetic analysis of reproductive development in tomato, 10.1387/ijdb.072440rl
  8. Périlleux, C., Lobet, G. & Tocquin, P. Inflorescence development in tomato: gene functions within a zigzag model. Front. Plant Sci. 5, 121 (2014).
  9. Lippman Zachary B, Cohen Oded, Alvarez John P, Abu-Abied Mohamad, Pekker Irena, Paran Ilan, Eshed Yuval, Zamir Dani, The Making of a Compound Inflorescence in Tomato and Related Nightshades, 10.1371/journal.pbio.0060288
  10. Park S. J., Jiang K., Schatz M. C., Lippman Z. B., Rate of meristem maturation determines inflorescence architecture in tomato, 10.1073/pnas.1114963109
  11. Thouet Johanna, Quinet Muriel, Lutts Stanley, Kinet Jean-Marie, Périlleux Claire, Repression of Floral Meristem Fate Is Crucial in Shaping Tomato Inflorescence, 10.1371/journal.pone.0031096
  12. Chetelat, R. T. Revised list of miscellaneous stocks. Tomato Genet. Cooperative Rep. 55, 48–69 (2005).
  13. Molinero-Rosales Nuria, Jamilena Manuel, Zurita Sergio, Gomez Pedro, Capel Juan, Lozano Rafael, FALSIFLORA, the tomato orthologue of FLORICAULA and LEAFY, controls flowering time and floral meristem identity, 10.1046/j.1365-313x.1999.00641.x
  14. Allen KeithD., Sussex IanM., Falsiflora and anantha control early stages of floral meristem development in tomato (Lycopersicon esculentum Mill.), 10.1007/bf00208316
  15. Quinet Muriel, Dubois Céline, Goffin Marie-Christine, Chao Jaime, Dielen Vincent, Batoko Henri, Boutry Marc, Kinet Jean-Marie, Characterization of tomato (Solanum lycopersicum L.) mutants affected in their flowering time and in the morphogenesis of their reproductive structure, 10.1093/jxb/erj117
  16. MacAlister Cora A, Park Soon Ju, Jiang Ke, Marcel Fabien, Bendahmane Abdelhafid, Izkovich Yinon, Eshed Yuval, Lippman Zachary B, Synchronization of the flowering transition by the tomato TERMINATING FLOWER gene, 10.1038/ng.2465
  17. BUTLER L., THE LINKAGE MAP OF THE TOMATO, 10.1093/oxfordjournals.jhered.a106252
  18. Szymkowiak E. J., Interactions between jointless and Wild-Type Tomato Tissues during Development of the Pedicel Abscission Zone and the Inflorescence Meristem, 10.1105/tpc.11.2.159
  19. Mao, L. et al. JOINTLESS is a MADS-box gene controlling tomato flower abscission zone development. Nature 206, 910–913 (2000).
  20. Molinero-Rosales Nuria, Latorre Antonio, Jamilena Manuel, Lozano Rafael, SINGLE FLOWER TRUSS regulates the transition and maintenance of flowering in tomato, 10.1007/s00425-003-1109-1
  21. Lifschitz E., Eviatar T., Rozman A., Shalit A., Goldshmidt A., Amsellem Z., Alvarez J. P., Eshed Y., The tomato FT ortholog triggers systemic signals that regulate growth and flowering and substitute for diverse environmental stimuli, 10.1073/pnas.0601620103
  22. Lifschitz E., Eshed Y., Universal florigenic signals triggered by FT homologues regulate growth and flowering cycles in perennial day-neutral tomato, 10.1093/jxb/erl106
  23. Vrebalov J., A MADS-Box Gene Necessary for Fruit Ripening at the Tomato Ripening-Inhibitor (Rin) Locus, 10.1126/science.1068181
  24. Nakano T., Kimbara J., Fujisawa M., Kitagawa M., Ihashi N., Maeda H., Kasumi T., Ito Y., MACROCALYX and JOINTLESS Interact in the Transcriptional Regulation of Tomato Fruit Abscission Zone Development, 10.1104/pp.111.183731
  25. Nakano Toshitsugu, Fujisawa Masaki, Shima Yoko, Ito Yasuhiro, Expression profiling of tomato pre-abscission pedicels provides insights into abscission zone properties including competence to respond to abscission signals, 10.1186/1471-2229-13-40
  26. Liu Danmei, Wang Di, Qin Zhengrui, Zhang Dongdong, Yin Lingjie, Wu Liang, Colasanti Joseph, Li Aili, Mao Long, The SEPALLATA MADS-box protein SLMBP21 forms protein complexes with JOINTLESS and MACROCALYX as a transcription activator for development of the tomato flower abscission zone, 10.1111/tpj.12387
  27. Campisi Lauren, Yang Yingzhen, Yi Ying, Heilig Elizabeth, Herman Benjamin, Cassista A. Jon, Allen David W., Xiang Hongjun, Jack Thomas, Generation of enhancer trap lines in Arabidopsis and characterization of expression patterns in the inflorescence, 10.1046/j.1365-313x.1999.00409.x
  28. Schupp, J. M., Price, L. B., Klevytska, A. & Keim, P. Internal and flanking sequence from AFLP fragments using ligation-mediated suppression PCR. Biotechniques 26, 905–912 (1999).
  29. Spertini, D., Béliveau, C. & Bellemare, G. Screening of transgenic plants by amplification of unknown genomic DNA flanking T-DNA. Biotechniques 27, 308–314 (1999).
  30. Szymkowiak Eugene J., Irish Erin E., JOINTLESS suppresses sympodial identity in inflorescence meristems of tomato, 10.1007/s00425-005-0115-x
  31. Shalit A., Rozman A., Goldshmidt A., Alvarez J. P., Bowman J. L., Eshed Y., Lifschitz E., The flowering hormone florigen functions as a general systemic regulator of growth and termination, 10.1073/pnas.0810810106
  32. Kaufmann K., Wellmer F., Muino J. M., Ferrier T., Wuest S. E., Kumar V., Serrano-Mislata A., Madueno F., Krajewski P., Meyerowitz E. M., Angenent G. C., Riechmann J. L., Orchestration of Floral Initiation by APETALA1, 10.1126/science.1185244
  33. Grandi Valentina, Gregis Veronica, Kater Martin M., Uncovering genetic and molecular interactions among floral meristem identity genes in Arabidopsis thaliana : Interactions among AGL24, SVP, AP1, LMI1 and LFY, 10.1111/j.1365-313x.2011.04840.x
  34. Teo Zhi Wei Norman, Song Shiyong, Wang Yong-Qiang, Liu Jie, Yu Hao, New insights into the regulation of inflorescence architecture, 10.1016/j.tplants.2013.11.001
  35. Ellul Philippe, Angosto Trinidad, García-Sogo Begoña, García-Hurtado Noemí, Martín-Trillo Mar, Salinas María, Moreno Vicente, Lozano Rafael, Martínez-Zapater José M., Expression of Arabidopsis APETALA1 in tomato reduces its vegetative cycle without affecting plant production, 10.1023/b:molb.0000018763.64585.6b
  36. Sun Lei-Ming, Zhang Jin-Zhi, Mei Li, Hu Chun-Gen, Molecular cloning, promoter analysis and functional characterization of APETALA 1-like gene from precocious trifoliate orange (Poncirus trifoliata L. Raf.), 10.1016/j.scienta.2014.08.015
  37. Quinet Muriel, Dielen Vincent, Batoko Henri, Boutry Marc, Havelange Andrée, Kinet Jean-Marie, Genetic interactions in the control of flowering time and reproductive structure development in tomato (Solanum lycopersicum), 10.1111/j.1469-8137.2006.01717.x
  38. Huijser, P. et al. Bracteomania, an inflorescence anomaly, is caused by the loss of function of the MADS-box gene squamosa in Antirrhinum majus. EMBO J. 11, 1239–1249 (1992).
  39. Irish V. F., Function of the apetala-1 Gene during Arabidopsis Floral Development, 10.1105/tpc.2.8.741
  40. Ditta Gary, Pinyopich Anusak, Robles Pedro, Pelaz Soraya, Yanofsky Martin F., The SEP4 Gene of Arabidopsis thaliana Functions in Floral Organ and Meristem Identity, 10.1016/j.cub.2004.10.028
  41. Atarés Alejandro, Moyano Elena, Morales Belén, Schleicher Peter, García-Abellán José Osvaldo, Antón Teresa, García-Sogo Begoña, Perez-Martin Fernando, Lozano Rafael, Flores Francisco Borja, Moreno Vicente, del Carmen Bolarin María, Pineda Benito, An insertional mutagenesis programme with an enhancer trap for the identification and tagging of genes involved in abiotic stress tolerance in the tomato wild-related species Solanum pennellii, 10.1007/s00299-011-1094-y
  42. Lozano Rafael, Angosto Trinidad, Gómez Pedro, Payán Carmen, Capel Juan, Huijser Peter, Salinas Julio, Martı́nez-Zapater José M., Tomato Flower Abnormalities Induced by Low Temperatures Are Associated with Changes of Expression of MADS-Box Genes, 10.1104/pp.117.1.91
  43. Dellaporta Stephen L., Wood Jonathan, Hicks James B., A plant DNA minipreparation: Version II, 10.1007/bf02712670
  44. Ausubel, F. M. et al. Preparation and analysis of DNA, In Current Protocols in Molecular Biology. (eds Ausubel, F. M. et al. .) Unit 2.2. (John Wiley and Sons, 1995).
  45. Workman Christopher, Jensen Lars, Jarmer Hanne, Berka Randy, Gautier Laurent, Nielser Henrik, Saxild Hans-Henrik, Nielsen Claus, Brunak Søren, Knudsen Steen, 10.1186/gb-2002-3-9-research0048
  46. Gentleman Robert C, Carey Vincent J, Bates Douglas M, Bolstad Ben, Dettling Marcel, Dudoit Sandrine, Ellis Byron, Gautier Laurent, Ge Yongchao, Gentry Jeff, Hornik Kurt, Hothorn Torsten, Huber Wolfgang, Iacus Stefano, Irizarry Rafael, Leisch Friedrich, Li Cheng, Maechler Martin, Rossini Anthony J, Sawitzki Gunther, Smith Colin, Smyth Gordon, Tierney Luke, Yang Jean YH, Zhang Jianhua, 10.1186/gb-2004-5-10-r80
Bibliographic reference Yuste-Lisbona, Fernando ; Quinet, Muriel ; Fernandez-Lozano, Antonia ; Pineda, Benito ; Moreno, Vincente ; et. al. Characterization of vegetative inflorescence (mc-vin) mutant provides new insight into the role of MACROCALYX in regulating inflorescence development of tomato.. In: Scientific Reports, Vol. 6, p. 18796 (2016)
Permanent URL http://hdl.handle.net/2078.1/170257