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Specific localization and measurement of hydrogen peroxide in Arabidopsis thaliana cell suspensions and protoplasts elicited by COS-OGA

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Ledoux, Quentin [UCL] Van Cutsem , Pierre [UCL] Marko, Istvan [UCL] Veys , Pascal [UCL]

H2O2 acts as an important signaling molecule during plant/pathogen interactions but its study remains a challenge due to the current shortcomings in H2O2-responsive probes. In this work, ContPY1, a new molecular probe developed to specifically detect H2O2 was used to study the elicitation of Arabidopsis thaliana cells by a complex of chitosan oligomers (COS) and oligogalacturonides (OGA). The comparison of cell suspensions, protoplasts of cell suspensions and leaf protoplasts treated with different inhibitors gave indications on the potential sources of hydrogen peroxide in plant cells. The relative contribution of the cell wall, of membrane dehydrogenases and of peroxidases depended on cell type and treatment and proved to be variable. Our present protocol can be used to study hydrogen peroxide production in a large variety of plant species by simple protocol adaptation.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2014
Language Anglais
Journal information "Plant Signalling & Behavior" - Vol. 9, no. 5, p. 1-6 (2014)
Peer reviewed yes
Publisher Landes Bioscience ((United States) Austin)
issn 1559-2316
e-issn 1559-2324
Publication status Publié
Affiliation UCL - SST/IMCN/MOST - Molecules, Solids and Reactivity
Keywords Arabidopsis ; Cell suspension ; elicitor ; hydrogen ; peroxide ; microscopy ; protoplast
Links
  1. Doke N., Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components, 10.1016/0048-4059(83)90019-x
  2. Henzler Tobias, Steudle Ernst, Transport and metabolic degradation of hydrogen peroxide in Chara corallina: model calculations and measurements with the pressure probe suggest transport of H2O2 across water channels, 10.1093/jexbot/51.353.2053
  3. Vera-Estrella R., Blumwald E., Higgins V. J., Effect of Specific Elicitors of Cladosporium fulvum on Tomato Suspension Cells : Evidence for the Involvement of Active Oxygen Species, 10.1104/pp.99.3.1208
  4. Somssich Imre E., Hahlbrock Klaus, Pathogen defence in plants — a paradigm of biological complexity, 10.1016/s1360-1385(98)01199-6
  5. Vianello Angelo, Macri Francesco, Generation of superoxide anion and hydrogen peroxide at the surface of plant cells, 10.1007/bf00771012
  6. Asada K., Production and Scavenging of Reactive Oxygen Species in Chloroplasts and Their Functions, 10.1104/pp.106.082040
  7. Davies D. R, Production of reactive oxygen species in Arabidopsis thaliana cell suspension cultures in response to an elicitor from Fusarium oxysporum: implications for basal resistance, 10.1093/jxb/erj216
  8. Corpas Francisco J, Barroso Juan B, del Rı́o Luis A, Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells, 10.1016/s1360-1385(01)01898-2
  9. Gómez-Gómez Lourdes, Boller Thomas, Flagellin perception: a paradigm for innate immunity, 10.1016/s1360-1385(02)02261-6
  10. O'Brien J. A., Daudi A., Finch P., Butt V. S., Whitelegge J. P., Souda P., Ausubel F. M., Bolwell G. P., A Peroxidase-Dependent Apoplastic Oxidative Burst in Cultured Arabidopsis Cells Functions in MAMP-Elicited Defense, 10.1104/pp.111.190140
  11. Ledoux Quentin, Veys Pascal, Van Cutsem Pierre, Mauro Sergio, Lucaccioni Fabio, Marko Istvan, Validation of the boronate sensor ContPY1 as a specific probe for fluorescent detection of hydrogen peroxide in plants, 10.4161/psb.26827
  12. Cabrera J. C., Boland A., Cambier P., Frettinger P., Van Cutsem P., Chitosan oligosaccharides modulate the supramolecular conformation and the biological activity of oligogalacturonides in Arabidopsis, 10.1093/glycob/cwq034
  13. Meunier Christophe F., Rooke Joanna C., Hajdu Kata, Van Cutsem Pierre, Cambier Pierre, Léonard Alexandre, Su Bao-Lian, Insight into Cellular Response of Plant Cells Confined within Silica-Based Matrices, 10.1021/la9039286
  14. Messiaen J, Plant Cell Physiol, 36, 1213 (1995)
  15. Gibeaut D. M., Hulett J., Cramer G. R., Seemann J. R., Maximal Biomass of Arabidopsis thaliana Using a Simple, Low-Maintenance Hydroponic Method and Favorable Environmental Conditions, 10.1104/pp.115.2.317
  16. Yoo Sang-Dong, Cho Young-Hee, Sheen Jen, Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis, 10.1038/nprot.2007.199
  17. Gamborg O.L., Miller R.A., Ojima K., Nutrient requirements of suspension cultures of soybean root cells, 10.1016/0014-4827(68)90403-5
  18. Hampton Mark B., Winterbourn Christine C., Modification of neutrophil oxidant production with diphenyleneiodonium and its effect on bacterial killing, 10.1016/0891-5849(94)00181-i
  19. Cross A R, Jones O T G, The effect of the inhibitor diphenylene iodonium on the superoxide-generating system of neutrophils. Specific labelling of a component polypeptide of the oxidase, 10.1042/bj2370111
  20. KHOKON MD. ATIQUR RAHMAN, OKUMA EIJI, HOSSAIN MOHAMMAD ANOWAR, MUNEMASA SHINTARO, URAJI MISUGI, NAKAMURA YOSHIMASA, MORI IZUMI C., MURATA YOSHIYUKI, Involvement of extracellular oxidative burst in salicylic acid-induced stomatal closure in Arabidopsis : Extracellular ROS mediate SA-induced stomatal closure, 10.1111/j.1365-3040.2010.02253.x
  21. Dickinson Bryan C., Chang Christopher J., A Targetable Fluorescent Probe for Imaging Hydrogen Peroxide in the Mitochondria of Living Cells, 10.1021/ja802355u
  22. Torres Miguel Angel, ROS in biotic interactions, 10.1111/j.1399-3054.2009.01326.x
  23. Zurbriggen Matias D., Carrillo Néstor, Hajirezaei Mohammad-Reza, ROS signaling in the hypersensitive response : When, where and what for?, 10.4161/psb.5.4.10793
  24. Frahry Gitta, Schopfer Peter, Inhibition of O2-reducing activity of horseradish peroxidase by diphenyleneiodonium, 10.1016/s0031-9422(98)00004-1
  25. Bolwell G. P., The apoplastic oxidative burst in response to biotic stress in plants: a three-component system, 10.1093/jexbot/53.372.1367
  26. Gutiérrez Jorge, González-Pérez Sergio, García-García Francisco, Lorenzo Óscar, Arellano Juan B., Does singlet oxygen activate cell death in Arabidopsis cell suspension cultures?: Analysis of the early transcriptional defense responses to high light stress, 10.4161/psb.6.12.18264
  27. Zurbriggen Matias D., Carrillo Néstor, Tognetti Vanesa B., Melzer Michael, Peisker Martin, Hause Bettina, Hajirezaei Mohammad-Reza, Chloroplast-generated reactive oxygen species play a major role in localized cell death during the non-host interaction between tobacco and Xanthomonas campestris pv. vesicatoria : Chloroplast ROS in a non-host interaction, 10.1111/j.1365-313x.2009.04010.x
  28. Allan A. C., Two Distinct Sources of Elicited Reactive Oxygen Species in Tobacco Epidermal Cells, 10.1105/tpc.9.9.1559
Bibliographic reference Ledoux, Quentin ; Van Cutsem , Pierre ; Marko, Istvan ; Veys , Pascal. Specific localization and measurement of hydrogen peroxide in Arabidopsis thaliana cell suspensions and protoplasts elicited by COS-OGA. In: Plant Signalling & Behavior, Vol. 9, no. 5, p. 1-6 (2014)
Permanent URL http://hdl.handle.net/2078.1/156606