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Determination of Bacillus cereus Emetic Toxin in Food Products by Means of LC-MS 2

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Delbrassinne, Laurence [Scientific Institute of Public Health, Brussel] Andjelkovic, Mirjana [Scientific Institute of Public Health, Brussel] Rajkovic, Andreja [Ghent University] Dubois, Pauline [Scientific Institute of Public Health, Brussel] N'guessan, Elise [UCL] Mahillon, Jacques [UCL] van Loco, Joris [Scientific Institute of Public Health, Brussel]

Cereulide is the heat-stable toxin produced by certain strains of Bacillus cereus. It is the main virulence factor of emetic B. cereus strains, which causes the emetic food poisoning syndrome, including rare fatal cases of food intoxications. Due to presumably low intoxication doses, a sensitive, specific, and robust technique is needed for its detection. In 2002, a LC-MS method was developed which allowed absolute quantification of cereulide using valinomycin as standard. This study describes the validation, according to the Commission Decision 2002/657/EC, of the LC-MS 2 method, a tandem mass spectrometry technique, which guarantees lower detection limit and higher specificity. The LC-MS 2 method, calibrated with valinomycin, was validated in rice and tested on various matrices (i. e., red beans, spices, and chili con carne) containing cereulide. The process combines a simple extraction step from the food matrix followed by LC-MS 2 analysis and detection by ion trap mass spectrometer. The detection limit for cereulide in rice was 0. 5 ng eq/g, which is 20 to 2,500 times lower than currently understood intoxicative doses between 10 and 1. 280 ng/g previously reported for cereulide. The validated method was specific, sensitive, repeatable, and reproducible with recoveries ranging from 77% to 101%. © 2011 Springer Science+Business Media, LLC.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2012
Language Anglais
Journal information "Food Analytical Methods" - Vol. 5, no. 5, p. 969-979 (2012)
Peer reviewed yes
Publisher Springer New York LLC ((United States) New York)
issn 1936-9751
e-issn 1936-976X
Publication status Publié
Affiliation UCL - SST/ELI/ELIM - Applied Microbiology
Keywords Cereulide ; Food matrices ; LC-MS 2 ; Method validation ; 3131
Links
  1. Agata N, Mori M, Ohta M, Suwan S, Ohtani I, Isobe M (1994) A novel dodecadepsipeptide, cereulide, isolated from Bacillus cereus causes vacuole formation in HEp-2 cells. FEMS Microbiol Lett 121:31–34
  2. Agata N, Ohta M, Mori M, Isobe M (1995) A novel dodecadepsipeptide, cereulide, is an emetic toxin of Bacillus cereus. FEMS Microbiol Lett 129:17–19
  3. Agata N, Ohta M, Yokoyama K, Production of Bacillus cereus emetic toxin (cereulide) in various foods, 10.1016/s0168-1605(01)00692-4
  4. Altayar M., Sutherland A.D., Bacillus cereus is common in the environment but emetic toxin producing isolates are rare, 10.1111/j.1365-2672.2005.02764.x
  5. Andersson MA, Mikkola R, Helin J, Andersson MC, Salkinoja-Salonen M (1998) A novel sensitive bioassay for detection of Bacillus cereus emetic toxin and related depsipeptide ionophores. Appl Environ Microbiol 64:1338–1343
  6. ANDERSSON M, Sperm bioassay for rapid detection of cereulide-producing Bacillus cereus in food and related environments, 10.1016/j.ijfoodmicro.2004.01.018
  7. Andersson Maria A., Hakulinen Pasi, Honkalampi-Hämäläinen Ulla, Hoornstra Douwe, Lhuguenot Jean-Claude, Mäki-Paakkanen Jorma, Savolainen Martti, Severin Isabelle, Stammati Anna-Laura, Turco Laura, Weber Assi, von Wright Atte, Zucco Flavia, Salkinoja-Salonen Mirja, Toxicological profile of cereulide, the Bacillus cereus emetic toxin, in functional assays with human, animal and bacterial cells, 10.1016/j.toxicon.2006.10.006
  8. Bauer Tobias, Stark Timo, Hofmann Thomas, Ehling-Schulz Monika, Development of a Stable Isotope Dilution Analysis for the Quantification of theBacillus cereusToxin Cereulide in Foods, 10.1021/jf9033046
  9. Beattie S.H., Williams A.G., BACILLUS | Detection of Toxins, Encyclopedia of Food Microbiology (1999) ISBN:9780122270703 p.141-149, 10.1006/rwfm.1999.0125
  10. Biesta-Peters E. G., Reij M. W., Blaauw R. H., in 't Veld P. H., Rajkovic A., Ehling-Schulz M., Abee T., Quantification of the Emetic Toxin Cereulide in Food Products by Liquid Chromatography-Mass Spectrometry Using Synthetic Cereulide as a Standard, 10.1128/aem.01659-10
  11. Ceuppens Siele, Rajkovic Andreja, Heyndrickx Marc, Tsilia Varvara, Van De Wiele Tom, Boon Nico, Uyttendaele Mieke, Regulation of toxin production by Bacillus cereus and its food safety implications, 10.3109/1040841x.2011.558832
  12. Delbrassinne Laurence, Andjelkovic Mirjana, Rajkovic Andreja, Bottledoorn Nadine, Mahillon Jacques, Van Loco Joris, Follow-up of the Bacillus cereus emetic toxin production in penne pasta under household conditions using liquid chromatography coupled with mass spectrometry, 10.1016/j.fm.2011.02.014
  13. Dierick K., Van Coillie E., Swiecicka I., Meyfroidt G., Devlieger H., Meulemans A., Hoedemaekers G., Fourie L., Heyndrickx M., Mahillon J., Fatal Family Outbreak of Bacillus cereus-Associated Food Poisoning, 10.1128/jcm.43.8.4277-4279.2005
  14. European Commission Decision 2002/657/EC (2002) Official Journal of the European Communities L221 8
  15. European Commission Decision 333/2007/EC (2007) Official Journal of the European Communities L88/35
  16. European Community Council Directive 93/88/EC (1993) Official Journal of the European Communities L268/71-82
  17. Fretz R., Schmid D., Brueller W., Girsch L., Pichler A.M., Riediger K., Safer M., Allerberger F., Food poisoning due to Jimson weed mimicking Bacillus cereus food intoxication in Austria, 2006, 10.1016/j.ijid.2007.03.003
  18. Granum Per Einar, Lund Terje, Bacillus cereus and its food poisoning toxins, 10.1111/j.1574-6968.1997.tb12776.x
  19. Granum PE, Baird-Parker TC (2000) Bacillus spp. In: Lund B, Baird-Parker TC, Gould G (eds) Microbiological safety and quality of food. Aspen, Frederick, pp 1029–1039
  20. Haggblom M. M, Apetroaie C., Andersson M. A., Salkinoja-Salonen M. S., Quantitative Analysis of Cereulide, the Emetic Toxin of Bacillus cereus, Produced under Various Conditions, 10.1128/aem.68.5.2479-2483.2002
  21. Hughes Stephanie, Bartholomew Barbara, Hardy J.C., Kramer J.M., Potential application of a HEp-2 cell assay in the investigation ofBacillus cereusemetic-syndrome food poisoning, 10.1111/j.1574-6968.1988.tb02563.x
  22. Horwitz W, Albert R (2006) The Horwitz ratio (HorRat): a useful index of method performance with respect to precision. J AOAC Int 89:1095–1109
  23. In’t Veld PH, Fitz-James IA, Nuppunen M, Kankare M, Johansson T (2010) Quantification of cereulide from food poisoning outbreaks caused by Bacillus cereus, Proceedings of FoodMicro Symposium, poster nb PEB1.26
  24. ISO 5725–2 (1994) Accuracy (trueness and precision) of measurement methods and results-basic method for the determination of repeatability and reproducibility of a standard measurement method
  25. Isobe Minoru, Ishikawa Tetsuya, Suwan Sathorn, Agata Norio, Ohta Michio, Synthesis and activity of cereulide, a cyclic dodecadepsipeptide ionophore as emetic toxin from Bacillus cereus, 10.1016/0960-894x(95)00503-l
  26. Jääskeläinen E.L, Teplova V, Andersson M.A, Andersson L.C, Tammela P, Andersson M.C, Pirhonen T.I, Saris N.-E.L, Vuorela P, Salkinoja-Salonen M.S, In vitro assay for human toxicity of cereulide, the emetic mitochondrial toxin produced by food poisoning Bacillus cereus, 10.1016/s0887-2333(03)00096-1
  27. JÄÄSKELÄINEN ELINA L., HÄGGBLOM MAX M., ANDERSSON MARIA A., VANNE LIISA, SALKINOJA-SALONEN MIRJA S., Potential of Bacillus cereus for Producing an Emetic Toxin, Cereulide, in Bakery Products: Quantitative Analysis by Chemical and Biological Methods, 10.4315/0362-028x-66.6.1047
  28. Jääskeläinen E.L., Häggblom M.M., Andersson M.A., Salkinoja-Salonen M.S., Atmospheric oxygen and other conditions affecting the production of cereulide by Bacillus cereus in food, 10.1016/j.ijfoodmicro.2004.03.011
  29. Kawamura-Sato Kumiko, Hirama Yumi, Agata Norio, Ito Hideo, Torii Kenzo, Takeno Akira, Hasegawa Tadao, Shimomura Yoshiharu, Ohta Michio, Quantitative Analysis of Cereulide, an Emetic Toxin ofBacillus cereus, by Using Rat Liver Mitochondria, 10.1111/j.1348-0421.2005.tb03636.x
  30. Magnus M (2008) Essential readings in infectious disease epidemiology. Jones & Bartlett Learning, Sudbury
  31. Mahler Hellmut, Pasi Aurelio, Kramer John M., Schulte Petra, Scoging Anne C., Bär Walter, Krähenbühl Stephan, Fulminant Liver Failure in Association with the Emetic Toxin ofBacillus cereus, 10.1056/nejm199704173361604
  32. Mandel J (1964) The statistical analysis of experimental data. Wiley, New York
  33. Mead Paul, Food-Related Illness and Death in the United States, 10.3201/eid0505.990502
  34. Melling J., Capel B.J., Characteristics ofBacillus cereusemetic toxin, 10.1111/j.1574-6968.1978.tb02849.x
  35. Nelms PK, Larson O, Barnes-Josiah D (1997) Time to B. cereus about hot chocolate. Public Health Rep 112:240–244
  36. Nissen H., Rosnes J.T., Brendehaug J., Kleiberg G.H., Safety evaluation of sous vide-processed ready meals, 10.1046/j.1472-765x.2002.01218.x
  37. PAANANEN A., MIKKOLA R., SARENEVA T., MATIKAINEN S., HESS M., ANDERSSON M., JULKUNEN I., SALKINOJA-SALONEN M. S., TIMONEN T., Inhibition of human natural killer cell activity by cereulide, an emetic toxin fromBacillus cereus, 10.1046/j.1365-2249.2002.01898.x
  38. Pirhonen T.I., Andersson M.A., Jääskeläinen E.L., Salkinoja-Salonen M.S., Honkanen-Buzalski T., Johansson T.M.-L., Biochemical and toxic diversity of Bacillus cereus in a pasta and meat dish associated with a food-poisoning case, 10.1016/j.fm.2004.04.002
  39. Rajkovic Andreja, Uyttendaele Mieke, Deley Wouter, Van Soom Ann, Rijsselaere Tom, Debevere Johan, Dynamics of boar semen motility inhibition as a semi-quantitative measurement of Bacillus cereus emetic toxin (Cereulide), 10.1016/j.mimet.2005.09.018
  40. Rajkovic Andreja, Uyttendaele Mieke, Debevere Johan, Computer aided boar semen motility analysis for cereulide detection in different food matrices, 10.1016/j.ijfoodmicro.2006.09.031
  41. Rajkovic A., Uyttendaele M., Vermeulen A., Andjelkovic M., Fitz-James I., in ‘t Veld P., Denon Q., Vérhe R., Debevere J., Heat resistance of Bacillus cereus emetic toxin, cereulide, 10.1111/j.1472-765x.2008.02350.x
  42. SCHOENI JEAN L., LEE WONG AMY C., Bacillus cereus Food Poisoning and Its Toxins, 10.4315/0362-028x-68.3.636
  43. SHAHEEN R, ANDERSSON M, APETROAIE C, SCHULZ A, EHLINGSCHULZ M, OLLILAINEN V, SALKINOJASALONEN M, Potential of selected infant food formulas for production of Bacillus cereus emetic toxin, cereulide, 10.1016/j.ijfoodmicro.2005.10.007
  44. Shinagawa K, Konuma H, Sekita H, Sugii S (1995) Emesis of rhesus monkeys induced by intragastric administration with the HEp-2 vacuolation factor (cereulide) produced by Bacillus cereus. FEMS Microbiol Lett 130:87–90
  45. Shiota M., Saitou K., Mizumoto H., Matsusaka M., Agata N., Nakayama M., Kage M., Tatsumi S., Okamoto A., Yamaguchi S., Ohta M., Hata D., Rapid Detoxification of Cereulide in Bacillus cereus Food Poisoning, 10.1542/peds.2009-2319
  46. Turnbull P.C.B., Bacillus cereus toxins, 10.1016/0163-7258(81)90026-7
  47. Willoughby R, Sheehan E, Mitrovich S (1998) A global view of LC–MS: how to solve your most challenging analytical problems. In: Global View Publishing First (ed). Chem-Space Associates Inc., Pittsburgh
Bibliographic reference Delbrassinne, Laurence ; Andjelkovic, Mirjana ; Rajkovic, Andreja ; Dubois, Pauline ; N'guessan, Elise ; et. al. Determination of Bacillus cereus Emetic Toxin in Food Products by Means of LC-MS 2. In: Food Analytical Methods, Vol. 5, no. 5, p. 969-979 (2012)
Permanent URL http://hdl.handle.net/2078.1/140727