Wauters, Georges
[UCL]
Goossens, V.
Janssens, Michèle
[UCL]
Vandepitte, J.
A new enrichment medium for the recovery of pathogenic Yersinia enterocolitica serogroup O:3 from naturally infected meat products based on three selective agents, Irgasan, ticarcillin, and potassium chlorate (ITC), was compared with several other one- or two-step enrichments. Y. enterocolitica serogroup O:3 was recovered from 96.5% of 29 pork tongues, 24% of 50 ground pork samples, 16% of 25 masseter muscle samples, and 61% of tonsils. ITC was by far the most sensitive method for the recovery of Y. enterocolitica O:3, especially from ground meat and masseter muscles, while cold and two-step enrichments yielded better results for nonpathogenic strains. Plating of ITC enrichments onto SS-deoxycholate-calcium agar gave overall better results than plating onto cefsulodin-Irgasan-novobiocin agar for serogroup O:3.
Bibliographic reference |
Wauters, Georges ; Goossens, V. ; Janssens, Michèle ; Vandepitte, J.. New enrichment method for isolation of pathogenic Yersinia enterocolitica serogroup O:3 from pork.. In: Applied and environmental microbiology, Vol. 54, no. 4, p. 851-4 (1988) |
Permanent URL |
http://hdl.handle.net/2078.1/13993 |