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The anti-inflammatory effect of a pomegranate husk extract on inflamed adipocytes and macrophages cultivated independently, but not on the inflammatory vicious cycle between adipocytes and macrophages.

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Winand, Julie [UCL] Schneider, Yves-Jacques [UCL]

Obese adipose tissues contain a higher proportion of inflamed macrophages than the normal adipose tissue. Adipocytes and macrophages are known to secrete pro-inflammatory markers that establish the systemic inflammation leading to metabolic complications. CCL-2 secreted by hypertrophied adipocytes attracts and activates macrophages in the adipose tissue. These cells, in turn, secrete TNFα and other pro-inflammatory molecules. The pomegranate husk extract and its phenolic constituents, punicalagin and ellagic acid, have exhibited an anti-inflammatory effect. In this study, we used an in vitro coculture system of 3T3-L1 murine adipocytes and RAW 264.7 macrophages to investigate the potential anti-inflammatory effects of these compounds on the vicious cycle between both cell types. The pomegranate husk extract presented an anti-inflammatory effect on the inflamed cells cultivated independently, as suggested by a decrease of (i) CCL-2 secretion by both cell types, (ii) adipocyte IL-6 expression and secretion, and (iii) macrophage TNFα secretion. Nevertheless and surprisingly, no anti-inflammatory effect was observed in coculture. Punicalagin, at the same concentration as that found in the pomegranate extract, had a more potent effect than the extract and in coculture; it reduced significantly the IL-6 secretion. Ellagic acid decreased TNFα and CCL-2 macrophage secretion, CCL-2 adipocyte secretion and, in coculture, it reduced IL-6 secretion and expression by adipocytes. These results indicate that the pomegranate husk extract has an anti-inflammatory action on adipocytes and macrophages but seems to be not able to reduce the inflammatory vicious cycle between both cells. Ellagitannin and punicalagin showed a better effect on inflammation suggesting that PHE will be a good candidate for more investigations.

Document type Article de périodique (Journal article) – Article de recherche
Publication date 2014
Language Anglais
Journal information "Food & function" - Vol. 5, no. 2, p. 310-318 (2014)
Publisher Royal Society of Chemistry (Cambridge)
issn 2042-6496
e-issn 2042-650X
Affiliations UCL - SST/ISV - Institut des sciences de la vie
UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology
Links
  1. W. H. O. (WHO) , Obesity and overweight – Fact sheet no. 311, http://www.who.int/mediacentre/factsheets/fs311/en/
  2. Ouchi Noriyuki, Parker Jennifer L., Lugus Jesse J., Walsh Kenneth, Adipokines in inflammation and metabolic disease, 10.1038/nri2921
  3. Kamei Nozomu, Tobe Kazuyuki, Suzuki Ryo, Ohsugi Mitsuru, Watanabe Taku, Kubota Naoto, Ohtsuka-Kowatari Norie, Kumagai Katsuyoshi, Sakamoto Kentaro, Kobayashi Masatoshi, Yamauchi Toshimasa, Ueki Kohjiro, Oishi Yumiko, Nishimura Satoshi, Manabe Ichiro, Hashimoto Haruo, Ohnishi Yasuyuki, Ogata Hitomi, Tokuyama Kumpei, Tsunoda Masaki, Ide Tomohiro, Murakami Koji, Nagai Ryozo, Kadowaki Takashi, Overexpression of Monocyte Chemoattractant Protein-1 in Adipose Tissues Causes Macrophage Recruitment and Insulin Resistance, 10.1074/jbc.m601284200
  4. Medjakovic Svjetlana, Jungbauer Alois, Pomegranate: a fruit that ameliorates metabolic syndrome, 10.1039/c2fo30034f
  5. Cerd� Bego�a, Llorach Rafael, Cer�n Jos� J., Esp�n Juan Carlos, Tom�s-Barber�n Francisco A., Evaluation of the bioavailability and metabolism in the rat of punicalagin, an antioxidant polyphenol from pomegranate juice, 10.1007/s00394-003-0396-4
  6. Seeram Navindra P., Lee Rupo, Heber David, Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punica granatum L.) juice, 10.1016/j.cccn.2004.04.029
  7. Hollebeeck Sylvie, Winand Julie, Hérent Marie-France, During Alexandrine, Leclercq Joëlle, Larondelle Yvan, Schneider Yves-Jacques, Anti-inflammatory effects of pomegranate (Punica granatum L.) husk ellagitannins in Caco-2 cells, an in vitro model of human intestine, 10.1039/c2fo10258g
  8. Tsai Shu-Huei, Lin-Shiau Shoei-Yn, Lin Jen-Kun, Suppression of nitric oxide synthase and the down-regulation of the activation of NFκB in macrophages by resveratrol, 10.1038/sj.bjp.0702357
  9. Suganami T., Tanimoto-Koyama K., Nishida J., Itoh M., Yuan X., Mizuarai S., Kotani H., Yamaoka S., Miyake K., Aoe S., Kamei Y., Ogawa Y., Role of the Toll-like Receptor 4/NF- B Pathway in Saturated Fatty Acid-Induced Inflammatory Changes in the Interaction Between Adipocytes and Macrophages, 10.1161/01.atv.0000251608.09329.9a
  10. Al-Muammar May Nasser, Khan Fozia, Obesity: The preventive role of the pomegranate (Punica granatum), 10.1016/j.nut.2011.11.013
  11. Baranowski Maria, Enns Jennifer, Blewett Heather, Yakandawala Uma, Zahradka Peter, Taylor Carla G., Dietary flaxseed oil reduces adipocyte size, adipose monocyte chemoattractant protein-1 levels and T-cell infiltration in obese, insulin-resistant rats, 10.1016/j.cyto.2012.04.004
  12. Yamashita A, Soga Y, Iwamoto Y, Asano T, Li Y, Abiko Y, Nishimura F, DNA microarray analyses of genes expressed differentially in 3T3-L1 adipocytes co-cultured with murine macrophage cell line RAW264.7 in the presence of the toll-like receptor 4 ligand bacterial endotoxin, 10.1038/ijo.2008.153
  13. Wu Dan, Ma Xiaofeng, Tian Weixi, Pomegranate husk extract, punicalagin and ellagic acid inhibit fatty acid synthase and adipogenesis of 3T3-L1 adipocyte, 10.1016/j.jff.2013.01.005
  14. Kurokawa Jun, Arai Satoko, Nakashima Katsuhiko, Nagano Hiromichi, Nishijima Akemi, Miyata Keishi, Ose Rui, Mori Mayumi, Kubota Naoto, Kadowaki Takashi, Oike Yuichi, Koga Hisashi, Febbraio Maria, Iwanaga Toshihiko, Miyazaki Toru, Macrophage-Derived AIM Is Endocytosed into Adipocytes and Decreases Lipid Droplets via Inhibition of Fatty Acid Synthase Activity, 10.1016/j.cmet.2010.04.013
  15. Fain J N, Bahouth S W, Madan A K, TNFα release by the nonfat cells of human adipose tissue, 10.1038/sj.ijo.0802594
  16. Yamashita Akiko, Soga Yoshihiko, Iwamoto Yoshihiro, Yoshizawa Sayuri, Iwata Hirotaka, Kokeguchi Susumu, Takashiba Shogo, Nishimura Fusanori, Macrophage-Adipocyte Interaction: Marked Interleukin-6 Production by Lipopolysaccharide**, 10.1038/oby.2007.305
  17. Hien Tu, Mediators Inflammation, 972629 (2012)
  18. Rotter Victoria, Nagaev Ivan, Smith Ulf, Interleukin-6 (IL-6) Induces Insulin Resistance in 3T3-L1 Adipocytes and Is, Like IL-8 and Tumor Necrosis Factor-α, Overexpressed in Human Fat Cells from Insulin-resistant Subjects, 10.1074/jbc.m301977200
  19. Maury E., Brichard S.M., Adipokine dysregulation, adipose tissue inflammation and metabolic syndrome, 10.1016/j.mce.2009.07.031
  20. Hontecillas Raquel, O'Shea Marianne, Einerhand Alexandra, Diguardo Margaret, Bassaganya-Riera Josep, Activation of PPAR γ and α by Punicic Acid Ameliorates Glucose Tolerance and Suppresses Obesity-Related Inflammation, 10.1080/07315724.2009.10719770
  21. Shiner Maayan, Fuhrman Bianca, Aviram Michael, Macrophage paraoxonase 2 (PON2) expression is up-regulated by pomegranate juice phenolic anti-oxidants via PPARγ and AP-1 pathway activation, 10.1016/j.atherosclerosis.2007.01.007
  22. Tontonoz Peter, Spiegelman Bruce M., Fat and Beyond: The Diverse Biology of PPARγ, 10.1146/annurev.biochem.77.061307.091829
  23. Okuno A, Tamemoto H, Tobe K, Ueki K, Mori Y, Iwamoto K, Umesono K, Akanuma Y, Fujiwara T, Horikoshi H, Yazaki Y, Kadowaki T, Troglitazone increases the number of small adipocytes without the change of white adipose tissue mass in obese Zucker rats., 10.1172/jci1235
  24. Choi K.C., Ryu O.H., Lee K.W., Kim H.Y., Seo J.A., Kim S.G., Kim N.H., Choi D.S., Baik S.H., Choi K.M., Effect of PPAR-α and -γ agonist on the expression of visfatin, adiponectin, and TNF-α in visceral fat of OLETF rats, 10.1016/j.bbrc.2005.08.203
  25. de Nigris Filomena, Balestrieri Maria Luisa, Williams-Ignarro Sharon, D’Armiento Francesco P., Fiorito Carmela, Ignarro Louis J., Napoli Claudio, The influence of pomegranate fruit extract in comparison to regular pomegranate juice and seed oil on nitric oxide and arterial function in obese Zucker rats, 10.1016/j.niox.2007.04.005
  26. Shema-Didi Lilach, Sela Shifra, Ore Liora, Shapiro Galina, Geron Ronit, Moshe Goldfeld, Kristal Batya, One year of pomegranate juice intake decreases oxidative stress, inflammation, and incidence of infections in hemodialysis patients: A randomized placebo-controlled trial, 10.1016/j.freeradbiomed.2012.05.013
Bibliographic reference Winand, Julie ; Schneider, Yves-Jacques. The anti-inflammatory effect of a pomegranate husk extract on inflamed adipocytes and macrophages cultivated independently, but not on the inflammatory vicious cycle between adipocytes and macrophages.. In: Food & function, Vol. 5, no. 2, p. 310-318 (2014)
Permanent URL http://hdl.handle.net/2078.1/139229