User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Comparative analysis of the genomes of mycobacteriophages infecting saprophytic and pathogenic mycobacteria.

Primary tabs

Coene, M. [UCL] De Kesel, Myriam [UCL] Hong, N. T. [UCL] Gheysen, A. [UCL] Jezierska-Anczukow, A. [Institute for Tuberculosis, Warsaw, Poland] Cocito, Carlo [UCL]

The genomes of a series of mycobacteriophages have been analyzed to disclose possible relationships between genetic characteristics and host range. The percent guanine-plus-cytosine in the DNA of 14 phages was found to be 34.4 to 47.5, as determined by a double-labelling procedure, which is unaffected by the presence of modified bases. The DNA of few mycobacteriophages yielded discordant values when the G + C content was estimated by buoyant density determination and by the double labelling procedure. This observation suggests the possible presence of modified bases in these genomes. The reduced susceptibility of viral DNAs to several restriction endonucleases is suggestive of the occurrence of both methyladenine and methylcytosine in the genome of all the mycobacteriophages studied. Heterologous annealing among the 14 DNAs analyzed yielded 6 hybridization groups. Within one group, the homology level among viral genomes was estimated by comparing the electrophoretic mobilities of restriction fragments: values of 0.8 to 1.3% base substitution have thus been found. A comparison of the genomic characteristics and host range of the mycobacteriophages analyzed suggests a possible relationship between restriction pattern, G + C content, crosshybridization level and host range.

Document type Article de périodique (Journal article) – Comparative Study, Journal Article
Access type Accès restreint
Publication date 1993
Journal information "Archives of virology" - Vol. 133, no. 1-2, p. 39-49 (1993)
Peer reviewed yes
issn 0304-8608
Publication status Publié
Affiliation UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
MESH Subject Base Composition ; DNA - genetics - metabolism ; DNA Restriction Enzymes - metabolism ; DNA, Viral - genetics - metabolism ; Genome, Viral ; Mycobacteriophages - genetics ; Mycobacterium ; Sequence Homology
Links
  1. Bradley DE (1967) Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev 31: 230?314
  2. Coene M, Cocito C (1985) A microanalytical procedure for determination of the base composition of DNA. Eur J Biochem 150: 475?479
  3. Coene M, Hoet Ph, Cocito C (1983) Physical map of phage 2C DNA: evidence for the existence of large redundant ends. Eur J Biochem 132: 69?75
  4. Drapier JC, Quetier F, Petit JF (1978) Characterization of mycobacteriophage ATCC 11759. Unusual physicochemical properties of its DNA. Eur J Biochem 91: 163?170
  5. Engel HWB (1978) Mycobacteriophages and phage typing. Ann Microbiol Inst Pasteur 129 A: 75?90
  6. Froman S, Will DW, Bogen E (1954) Bacteriophage active against virulentMycobacterium tuberculosis. 1. Isolation and activity. Am J Public Health 44: 1325?1333
  7. Gardner GM, Weiser RS (1947 a). Discovery of a bacteriophage forMycobacterium smegmatis. J Bacteriol 54: 272?273
  8. Gardner GM, Weiser RS (1947 b). A bacteriophage forMycobacterium smegmatis. Proc Soc Exp Biol Med 66: 205?206
  9. Hoet Ph, Coene M, Cocito C (1983) Comparison of the physical maps and redundant ends of the chromosomes of phages 2C, SPOI, SP82, and ?e. Eur J Biochem 132: 63?67
  10. Hoet Ph, Coene M, Cocito C (1992) Replication cycle ofBacillus subtilis hydroxy-methyluracil-containing phages. Annu Rev Microbiol 46: 95?116
  11. Jacobs WR, Tuckman M, Bloom RB (1987) Introduction of foreign DNA into mycobacteria using a shuttle plasmid. Nature 327: 532?535
  12. Jones WD, Greenberg J (1977) Host modification and restriction with a mycobacteriophage isolated from a pseudolysogenicMycobacterium chelonei. J Gen Microbiol 99: 389?395
  13. Kessler C, Neumair PS, Wolf W (1985) Recognition sequences of restriction endonucleases and methylases - a review. Gene 33: 1?102
  14. Kraiss JP, Gelbart SM, Jukasz SE (1973) A comparison of three mycobacteriophages. J Gen Virol 20: 75?87
  15. Menezes J, Pavilanis V (1969) Properties of mycobacteriophage C2. Experientia 25: 1112?1113
  16. Rado TA, Bates JH (1980) Mycobacteriophage structure and function: a review. Adv Tubercul Res 20: 64?91
  17. Reddy AB, Gopinathan KP (1987) Characterization of mycobacteriophage 18 and its unrelatedness to mycobacteriophages I1, I3, and I15. J Gen Virol 68: 949?956
  18. Rigby PWJ, Dieckman M, Rhodes C, Berg P (1977) Labeling deoxynucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113: 237?251
  19. Sellers MI, Nakamura R, Tokunaga T (1970) The effects of ultra-violet radiation on mycobacteriophages and their infectious DNAs. J Gen Virol 20: 75?87
  20. Snapper SB, Lugosi L, Jekkel A, Melton RE, Kieser T, Bloom BR, Jacobs WR (1988) Lysogeny and transformation in mycobacteria: stable expression of foreign genes. Proc Natl Acad Sci USA 85: 6987?6991
  21. Soloff BL, Rado TA, Henry BE, Bates JH (1978) Biochemical and morphological characterization of mycobacteriophage R1. J Virol 25: 253?262
  22. Somogyi PA, Maso Bel M, Földes I (1982) Methylated nucleic acid bases inMycobacterium and mycobacteriophage DNA. Acta Microbiol Hung 29: 181?185
  23. Upholt WB (1977) Estimation of DNA sequence divergence from comparison of restriction endonuclease digests. Nucleic Acids Res 4: 1257?1265
  24. Warren RAJ (1980) Modified bases in bacteriophage DNAs. Annu Rev Microbiol 34: 137?158
Bibliographic reference Coene, M. ; De Kesel, Myriam ; Hong, N. T. ; Gheysen, A. ; Jezierska-Anczukow, A. ; et. al. Comparative analysis of the genomes of mycobacteriophages infecting saprophytic and pathogenic mycobacteria.. In: Archives of virology, Vol. 133, no. 1-2, p. 39-49 (1993)
Permanent URL http://hdl.handle.net/2078.1/13604