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Subtypes of Madin-Darby canine kidney (MDCK) cells defined by immunocytochemistry : further evidence for properties of renal collecting duct cells

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Devuyst, Olivier [UCL] Beauwens, Renaud [UCL] Denef, Jean-François [UCL] Crabbé, Jean [UCL] Abramow, Maurice [UCL]

The Madin-Darby canine kidney (MDCK) cell line has been proposed as a model for studying intercalated (IC) cells of the renal cortical collecting duct. The IC cells are characterized by peanut lectin (PNA) binding capacity, carbonic anhydrase (CA) activity and Cl(-)-HCO3- exchange mediated by a band 3-related protein. It has been suggested that these properties are also expressed in MDCK cells. So far however, the nature of the specific protein involved in Cl(-)-HCO3- exchange, the type of CA isozyme and the relationship between these two characteristics and PNA binding, have not been investigated in MDCK cells by immunocytochemical methods. Using two antibodies raised against human erythrocyte band 3 protein and two against human erythrocyte CA I and II isozymes, our study provides evidence that a protein related to band 3 is expressed in about 5% of cultured MDCK cells; these band 3-positive cells do not bind PNA and are not reactive for CAI or CAII. About 30% of the MDCK cells bind PNA, two-thirds of which are also CAII-positive. A majority (about 65%) of MDCK cells is not reactive for the three markers used; their density is increased after incubation with aldosterone. These data indicate (i) that the Cl(-)-HCO3- exchange of the MDCK cells could be related to human erythrocyte band 3, (ii) that the CA activity of the MDCK cell line bears antigenic identity with the erythrocyte CA II isozyme and (iii) that the latter is always co-localized with PNA binding.(ABSTRACT TRUNCATED AT 250 WORDS)

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 1994
Journal information "Cell and Tissue Research" - Vol. 277, no. 2, p. 231-237 (1994)
Peer reviewed yes
Publisher Springer (Heidelberg)
issn 0302-766X
e-issn 1432-0878
Publication status Publié
Affiliations UCL - MD/MINT - Département de médecine interne
UCL - (SLuc) Service de néphrologie
UCL - MD/MNOP - Département de morphologie normale et pathologique
MESH Subject Animals ; Anion Exchange Protein 1, Erythrocyte - metabolism ; Antiporters - metabolism ; Bicarbonates - metabolism ; Biological Markers ; Carbonic Anhydrases - metabolism ; Cell Line ; Chloride-Bicarbonate Antiporters ; Chlorides - metabolism ; Dogs ; Fluorescent Antibody Technique ; Immunohistochemistry ; Isoenzymes - metabolism ; Kidney Tubules, Collecting - cytology - metabolism ; Lectins - metabolism ; Peanut Agglutinin
Keywords Madin-Darby kidney (MDCK) cells ; Intercalated cells ; Collecting duct ; Renal ; Band 3 ; Human erythrocyte ; Carbonic anhydrase ; Peanut lectin
Links
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Bibliographic reference Devuyst, Olivier ; Beauwens, Renaud ; Denef, Jean-François ; Crabbé, Jean ; Abramow, Maurice. Subtypes of Madin-Darby canine kidney (MDCK) cells defined by immunocytochemistry : further evidence for properties of renal collecting duct cells. In: Cell and Tissue Research, Vol. 277, no. 2, p. 231-237 (1994)
Permanent URL http://hdl.handle.net/2078.1/13433