Antibacterial and antioxidant activity of three compounds isolated from Mitracarpus scaber

The antibacterial and the free radical scavenging activities of the alcoholic extract, total alkaloid extract and three compounds isolated from Mitracarpus scaber were evaluated using the p-iodonitrotetrazolium method and the DPPH method. The extracts and the three compounds identified as methoxy-4-acetophenon, 3,4,5-trimethoxybenzoic acid, azaanthraquinone were tested in vitro against five micro organisms. Azaanthraquinone showed the most interesting activity with minimum inhibitory concentrations values of 7.5 μg/ml, 19 μg/ml, 38 μg/ml and 150 μg/ml on Dermatophilus congolensis, Staphylococcus aureus, Enteroccocus feacalis, Escherichia coli and Pseudomonas aeruginosa, respectively. On the other hand, the three tested compounds showed mild scavenging activity in the DPPH test, with IC50 values between 12 μg/ml and 45.74 μg/ml.