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Atomic force microscopy and chemical force microscopy of microbial cells.

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Dufrêne, Yves [UCL]

Over the past years, atomic force microscopy (AFM) has emerged as a powerful tool for imaging the surface of microbial cells with nanometer resolution, and under physiological conditions. Moreover, chemical force microscopy (CFM) and single-molecule force spectroscopy have enabled researchers to map chemical groups and receptors on cell surfaces, providing valuable insight into their structure-function relationships. Here, we present protocols for analyzing spores of the pathogen Aspergillus fumigatus using real-time AFM imaging and CFM. We emphasize the use of porous polymer membranes for immobilizing single live cells, and the modification of gold-coated tips with alkanethiols for CFM measurements. We also discuss recording conditions and data interpretation, and provide recommendations for reliable experiments. For well-trained AFM users, the entire protocol can be completed in 2-3 d.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2008
Language Anglais
Journal information "Nature protocols" - Vol. 3, no. 7, p. 1132-8 (2008)
Peer reviewed yes
e-issn 1750-2799
issn 1754-2189
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Spores, Fungal - ultrastructure ; Microscopy, Atomic Force - methods ; Membranes, Artificial ; Aspergillus fumigatus - ultrastructure
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Bibliographic reference Dufrêne, Yves. Atomic force microscopy and chemical force microscopy of microbial cells.. In: Nature protocols, Vol. 3, no. 7, p. 1132-8 (2008)
Permanent URL http://hdl.handle.net/2078.1/12731