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Role of SycD, the chaperone of the Yersinia Yop translocators YopB and YopD.

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Neyt, C Cornelis, Guy

Extracellular Yersinia adhering at the surface of a eukaryotic cell translocate effector Yops across the plasma membrane of the cell by a mechanism requiring YopD and YopB, the latter probably mediating pore formation. We studied the role of SycD, the intrabacterial chaperone of YopD. By producing GST-YopB hybrid proteins and SycD in Escherichia coli, we observed that SycD also binds specifically to YopB and that this binding reduces the toxicity of GST-YopB in E. coli. By analysis of a series of truncated GST-YopB proteins, we observed that SycD does not bind to a discrete segment of YopB. Using the same approach, we observed that YopD can also bind to YopB. Binding between YopB and YopD occurred even in the presence of SycD, and a complex composed of these three proteins could be immunoprecipitated from the cytoplasm of Yersinia. In a sycD mutant, the intracellular pool of YopB and YopD was greatly reduced unless the lcrV gene was also deleted. As LcrV is known to interact with YopB and YopD and to promote their secretion, we speculate that SycD prevents a premature association between YopB-YopD and LcrV.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1999
Journal information "Molecular microbiology" - Vol. 31, no. 1, p. 143-56 (1999)
Peer reviewed yes
issn 0950-382X
Publication status Publié
Affiliation UCL
MESH Subject Antigens, Bacterial - metabolism ; Bacterial Outer Membrane Proteins - genetics - metabolism ; Bacterial Proteins - genetics - metabolism - physiology ; Binding Sites ; Molecular Chaperones - genetics - metabolism - physiology ; Pore Forming Cytotoxic Proteins ; Recombinant Fusion Proteins - genetics - metabolism ; Yersinia enterocolitica - genetics - metabolism
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Bibliographic reference Neyt, C ; Cornelis, Guy. Role of SycD, the chaperone of the Yersinia Yop translocators YopB and YopD.. In: Molecular microbiology, Vol. 31, no. 1, p. 143-56 (1999)
Permanent URL http://hdl.handle.net/2078.1/12530