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Fibronectin adsorption, conformation, and orientation on polystyrene substrates studied by radiolabeling, XPS, and ToF SIMS.

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Lhoest, J B Detrait, E van den Bosch de Aguilar, P Bertrand, Patrick [UCL]

Protein adsorption is widely studied by a variety of techniques, but there still is little known about protein orientation and conformation after adsorption. This probably is due to the large number of parameters involved, such as the characteristics of the surface and the structure of the protein. In this study, the adsorption of fibronectin was investigated with three different techniques: radiolabeling, X-ray photoelectron spectroscopy (XPS), and time-of-flight secondary ion mass spectrometry (ToF SIMS) on polystyrene and oxidized polystyrene. The first two techniques have been widely used to study protein adsorption, allowing us to determine the amount of protein adsorbed on each surface. The ToF SIMS, however, is a technique just emerging for the study of protein adsorption. This study confirms its utility since ToF SIMS is found to be sensitive to the protein orientation and/or conformation at the surface. Indeed, the ToF SIMS peaks characteristic of the protein show differences in their reduced intensity between the two substrates. These differences, which are not detected by XPS, are attributed to different orientations and/or conformations of the protein.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Publication date 1998
Journal information "Journal of biomedical materials research" - Vol. 41, no. 1, p. 95-103 (1998)
issn 0021-9304
Publication status Publié
Affiliation UCL
MESH Subject Adsorption ; Animals ; Cattle ; Fibronectins - metabolism ; Mass Spectrometry - methods ; Polystyrenes - chemistry ; Protein Conformation ; Solutions ; Spectrometry, X-Ray Emission ; Tritium
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Bibliographic reference Lhoest, J B ; Detrait, E ; van den Bosch de Aguilar, P ; Bertrand, Patrick. Fibronectin adsorption, conformation, and orientation on polystyrene substrates studied by radiolabeling, XPS, and ToF SIMS.. In: Journal of biomedical materials research, Vol. 41, no. 1, p. 95-103 (1998)
Permanent URL http://hdl.handle.net/2078.1/12446