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Baculovirus-infected cells do not produce the amyloid peptide of Alzheimer's disease from its precursor.

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Essalmani, R. Guillaume, J. M. Mercken, L. Octave, Jean-Noël [UCL]

The amyloid peptide (Abeta) of Alzheimer's disease (AD) is produced by proteolytic cleavage of a larger precursor, the amyloid peptide precursor or APP. The discovery of pathogenic mutations in the APP gene provides strong evidence for the hypothesis that APP metabolism is involved in the etiology of AD. To study the metabolism of the protein, human APP has been expressed in several mammalian cell types. Insect cells, infected by a recombinant baculovirus carrying the human APP sequence, also provide an interesting expression system because these cells do not produce endogenous APP. Baculovirus-infected cells synthesize very high amounts of extracellular soluble APP, after cleavage of the transmembrane protein, as described for mammalian cells. However, we demonstrate here that insect cells do not produce Abeta from APP. These results suggest that while the enzymatic activity needed for the production of soluble APP is conserved between insect and mammalian cells, the enzymes required for the production of Abeta from APP are only expressed in mammalian cells.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1996
Journal information "FEBS letters" - Vol. 389, no. 2, p. 157-61 (1996)
Peer reviewed yes
issn 0014-5793
Publication status Publié
Affiliation UCL - MD/FSIO - Département de physiologie et pharmacologie
MESH Subject Alzheimer Disease - metabolism ; Amino Acid Sequence ; Amyloid beta-Protein - biosynthesis - genetics ; Amyloid beta-Protein Precursor - genetics - metabolism ; Animals ; Baculoviridae - genetics ; Blotting, Western ; CHO Cells - metabolism ; Cricetinae ; Culture Media ; Endocytosis ; Genetic Vectors ; Humans ; Precipitin Tests ; Recombinant Proteins - biosynthesis - genetics ; Spodoptera - genetics - metabolism - virology
Links
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Bibliographic reference Essalmani, R. ; Guillaume, J. M. ; Mercken, L. ; Octave, Jean-Noël. Baculovirus-infected cells do not produce the amyloid peptide of Alzheimer's disease from its precursor.. In: FEBS letters, Vol. 389, no. 2, p. 157-61 (1996)
Permanent URL http://hdl.handle.net/2078.1/12210