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Study in vitro of the impact of endophytic bacteria isolated from Centella asiatica on the disease incidence caused by the hemibiotrophic fungus Colletotrichum higginsianum

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Rakotoniriana, Erick Francisco S. [UCL] Rafamantanana, Mamy [Institut malgache de recherches appliquées, Antananarivo, Madagascar] Randriamampionona, Denis [Institut malgache de recherches appliquées, Antananarivo, Madagascar] Rabemanantsoa, Christian [Institut malgache de recherches appliquées, Antananarivo, Madagascar] Urveg-Ratsimamanga, Suzanne [Institut malgache de recherches appliquées, Antananarivo, Madagascar] Munaut, Françoise [UCL] Corbisier, Anne-Marie [UCL] Quetin-Leclercq, Joëlle [UCL] Declerck, Stephan [UCL]

Thirty-one endophytic bacteria isolated from healthy leaves of Centella asiatica were screened in vitro for their ability to reduce the growth rate and disease incidence of Colletotrichum higginsianum, a causal agent of anthracnose. Isolates of Cohnella sp., Paenibacillus sp. and Pantoea sp. significantly stimulated the growth rate of C. higginsianum MUCL 44942, while isolates of Achromobacter sp., Acinetobacter sp., Microbacterium sp., Klebsiella sp. and Pseudomonas putida had no influence on this plant pathogen. By contrast, Bacillus subtilis BCA31 and Pseudomonas fluorescens BCA08 caused a marked inhibition of C. higginsianum MUCL 44942 growth by 46 and 82 %, respectively. Cell-free culture filtrates of B. subtilis BCA31 and P. fluorescens BCA08 were found to contain antifungal compounds against C. higginsianum MUCL 44942. Inoculation assays on in vitro-cultured plants of C. asiatica showed that foliar application of B. subtilis BCA31, three days before inoculation with C. higginsianum MUCL 44942, significantly reduced incidence and severity of the disease. The role of endophytic bacteria in maintaining the apparent inactivity of C. higginsianum MUCL 44942 in C. asiatica grown in the wild is discussed.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2013
Language Anglais
Journal information "Antonie van Leeuwenhoek : international journal of general and molecular microbiology" - Vol. 103, no.1, p. 121-133 (2013)
Peer reviewed yes
Publisher Springer Netherlands ((Netherlands) Dordrecht)
issn 0003-6072
e-issn 1572-9699
Publication status Publié
Affiliations UCL - SST/ELI/ELIM - Applied Microbiology
UCL - SSS/LDRI - Louvain Drug Research Institute
Keywords Antifungal activity ; Centella asiatica ; Colletotrichum higginsianum ; Endophytic bacteria ; n vitro plants ; 3130
Links
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Bibliographic reference Rakotoniriana, Erick Francisco S. ; Rafamantanana, Mamy ; Randriamampionona, Denis ; Rabemanantsoa, Christian ; Urveg-Ratsimamanga, Suzanne ; et. al. Study in vitro of the impact of endophytic bacteria isolated from Centella asiatica on the disease incidence caused by the hemibiotrophic fungus Colletotrichum higginsianum. In: Antonie van Leeuwenhoek : international journal of general and molecular microbiology, Vol. 103, no.1, p. 121-133 (2013)
Permanent URL http://hdl.handle.net/2078.1/121102