Buc Calderon, Pedro
[UCL]
Roberfroid, Marcel
[UCL]
N-phenylacetyl dehydroalanines are captodative olefins. They inhibit two processes mediated by superoxide anion (O2-.) in a concentration dependent manner: reduction of NBT to blue formazan and oxidation of epinephrine to adrenochrome. They also inhibit in a dose related way the degradation of deoxyribose produced during either the Fenton reaction or the radiolysis of water, which are the two experimental sources of hydroxyl radical (HO.) production. Based on the results obtained with superoxide dismutase, mannitol, thiourea, and uric acid, we postulate that these competitive inhibitory effects suggest a reaction between the dehydroalanine derivatives and the two oxygen derived radicals. Hydroxyl free radical is scavenged more efficiently than superoxide anion. Substitution of the phenyl ring by methoxy groups does not modify significantly the activity. These molecules possess three target active sites which can react with free radicals.
Bibliographic reference |
Buc Calderon, Pedro ; Roberfroid, Marcel. Inhibition of O2-.- and HO.- mediated processes by a new class of free radical scavengers: the N-acyl dehydroalanines.. In: Free radical research communications, Vol. 5, no. 3, p. 159-168 (1988) |
Permanent URL |
http://hdl.handle.net/2078.1/11695 |