User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Identification of a cluster IV pleiotropic drug resistance transporter gene expressed in the style of Nicotiana plumbaginifolia.

Primary tabs

Trombik, Tomasz [UCL] Jasinski, Michal Crouzet, Jérome Boutry, Marc [UCL]

ATP-binding cassette transporters of the pleiotropic drug resistance (PDR) subfamily are composed of five clusters. We have cloned a gene, NpPDR2, belonging to the still uncharacterized cluster IV from Nicotiana plumbaginifolia. NpPDR2 transcripts were found in the roots and mature flowers. In the latter, NpPDR2 expression was restricted to the style and only after pollination. A 1.5-kb genomic sequence containing the putative NpPDR2 transcription promoter was fused to the beta-glucuronidase reporter gene. The GUS expression pattern confirmed the RT-PCR results that NpPDR2 was expressed in roots and the flower style and showed that it was localized around the conductive tissues. Unlike other PDR genes, NpPDR2 expression was not induced in leaf tissues by none of the hormones typically involved in biotic and abiotic stress response. Moreover, unlike NpPDR1 known to be involved in biotic stress response, NpPDR2 expression was not induced in the style upon Botrytis cinerea infection. In N. plumbaginifolia plants in which NpPDR2 expression was prevented by RNA interference, no unusual phenotype was observed, including at the flowering stage, which suggests that NpPDR2 is not essential in the reproductive process under the tested conditions.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2008
Language Anglais
Journal information "Plant molecular biology" - Vol. 66, no. 1-2, p. 165-75 (2008)
Peer reviewed yes
issn 0167-4412
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Tobacco - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Molecular Sequence Data ; Genes, Plant ; Gene Expression Profiling ; Drug Resistance - genetics ; DNA, Complementary ; DNA Primers ; Base Sequence
Links
  1. Barth C, De Tullio M, Conklin PL (2006) The role of ascorbic acid in the control of flowering time and the onset of senescence. J Exp Bot 57:1657–1665
  2. Bell J, Hicks G (1976) Transmitting tissue in the pistil of tobacco: light and electron microscopic observations. Planta 131:187–200
  3. Boutry M, Chua N-H (1985) A nuclear gene encoding the beta subunit of the mitochondrial ATP synthase in Nicotiana plumbaginifolia. EMBO J 4:2159–2165
  4. Campbell EJ, Schenk PM, Kazan K, Penninckx I, Anderson JP, Maclean DJ, Cammue BPA, Ebert PR, Manners JM (2003) Pathogen-responsive expression of a putative ATP-binding cassette transporter gene conferring resistance to the diterpenoid sclareol is regulated by multiple defense signaling pathways in Arabidopsis. Plant Physiol 133:1272–1284
  5. Cheung AY, Wang H, Wu HM (1995) A floral transmitting tissue-specific glycoprotein attracts pollen tubes and stimulates their growth. Cell 82:383–393
  6. Crouzet J, Trombik T, Fraysse AS, Boutry M (2006) Organization and function of the plant pleiotropic drug resistance ABC transporter family. FEBS Lett 580:1123–1130
  7. Ducos E, Fraysse AS, Boutry M (2005) NtPDR3, an iron-deficiency inducible ABC transporter in Nicotiana tabacum. FEBS Lett 579:6791–6795
  8. Edlund AF, Swanson R, Preuss D (2004) Pollen and stigma structure and function: the role of diversity in pollination. Plant cell 16:S84–S97
  9. Eichhorn H, Klinghammer M, Becht P, Tenhaken R (2006) Isolation of a novel ABC-transporter gene from soybean induced by salicylic acid. J Exp Bot 57:2193–2201
  10. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:95–105
  11. van der Fits L, Deakin EA, Hoge JHC, Memelink J (2000) The ternary transformation system: constitutive virG on a compatible plasmid dramatically increases Agrobacterium-mediated plant transformation. Plant Mol Biol 43:495–502
  12. Gleave AP (1992) A versatile binary vector system with a T-DNA organizational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203–1207
  13. Goderis IJWM, Bolle MFCD, Francois IEJA, Wouters PFJ, Broekaert WF, Cammue BPA (2002) A set of modular plant transformation vectors allowing flexible insertion of up to six expression units. Plant Mol Biol 50:17–27
  14. Grec S, Vanham D, de Ribaucourt JC, Purnelle B, Boutry M (2003) Identification of regulatory sequence elements within the transcription promoter region of NpABC1, a gene encoding a plant ABC transporter induced by diterpenes. Plant J 35:237–250
  15. Higgins CF (2001) ABC transporters: physiology, structure and mechanism – an overview. Res Microbiol 152:205–210
  16. Holland IB, Blight AM (1999) ABC-ATPases, adaptable energy generators fuelling transmembrane movement of a variety of molecules in organisms from bacteria to humans. J Mol Biol 293:381–399
  17. Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers HJ, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231
  18. Ito H, Gray WM (2006) A gain-of-function mutation in the Arabidopsis pleiotropic drug resistance transporter PDR9 confers resistance to auxinic herbicides. Plant Physiol 142:63–74
  19. Jasinski M, Stukkens Y, Degand H, Purnelle B, Marchand-Brynaert J, Boutry M (2001) A plant plasma membrane ATP binding cassette-type transporter is involved in antifungal terpenoid secretion. Plant Cell 13:1095–1107
  20. Kim DY, Bovet L, Maeshima M, Martinoia E, Lee Y (2007) The ABC transporter AtPDR8 is a cadmium extrusion pump conferring heavy metal resistance. Plant J 50:207–218
  21. Kim S, Mollet JC, Dong J, Zhang KL, Park SY, Lord EM (2003) Chemocyanin, a small basic protein from the lily stigma, induces pollen tube chemotropism. Proc Natl Acad Sci U S A 100:16125–16130
  22. Kobae Y, Sekino T, Yoshioka H, Nakagawa T, Martinoia E, Maeshima M (2006) Loss of AtPDR8, a plasma membrane ABC transporter of Arabidopsis thaliana, causes hypersensitive cell death upon pathogen infection. Plant Cell Physiol 47:309–318
  23. Lee M, Lee K, Lee J, Noh EW, Lee Y (2005) AtPDR12 contributes to lead resistance in Arabidopsis. Plant Physiol 138:827–836
  24. Lord EM, Russell SD (2002) The mechanisms of pollination and fertilization in plants. Annu Rev Cell Dev Biol 18:81–105
  25. Lukaszewicz M, Feuermann M, Jerouville B, Stas A, Boutry M (2000) In vivo evaluation of the context sequence of the translation initiation codon in plants. Plant Sci 154:89–98
  26. Martinoia E, Klein M, Geisler M, Bovet L, Forestier C, Kolukisaoglu U, Müller-Röber B, Schulz B (2002) Multifunctionality of plant ABC transporters – more than just detoxifiers. Planta 214:345–355
  27. Moons A (2003) Ospdr9, which encodes a PDR-type ABC transporter, is induced by heavy metals, hypoxic stress and redox perturbations. FEBS Lett 553:370–376
  28. Ngugi HK, Scherm H (2004) Pollen mimicry during infection of blueberry flowers by conidia of Monilinia vaccinii-corymbosi. Physiol Mol Plant Pathol 64:113–123
  29. Ngugi HK, Scherm H (2006) Mimicry in plant-parasitic fungi. FEMS Microbiol Lett 257:171–176
  30. O’Brien M, Chantha SC, Rahier A, Matton DP (2005) Lipid signaling in plants. Cloning and expression analysis of the obtusifoliol 14 alpha-demethylase from Solanum chacoense Bitt., a pollination- and fertilization-induced gene with both obtusifoliol and lanosterol demethylase activity. Plant Physiol 139:734–749
  31. Ochmann H, Gerber AS, Hartl DL (1988) Genetic applications of an inverse polymerase chain reaction. Genetics 120:621–623
  32. Otsu CT, daSilva I, de Molfetta JB, da Silva LR, de Almeida-Engler J, Engler G, Torraca PC, Goldman GH, Goldman MHS (2004) NtWBC1, an ABC transporter gene specifically expressed in tobacco reproductive organs. J Exp Bot 55:1643–1654
  33. Palanivelu R, Brass L, Edlund AF, Preuss D (2003) Pollen tube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels. Cell 114:47–59
  34. Rea PA (2007) Plant ATP-binding cassette transporters. Annu Rev Plant Biol 58:347–375
  35. Rogers HJ (2006) Programmed cell death in floral organs: how and why do flowers die? Ann Bot 97:309–315
  36. Rubinstein B (2000) Regulation of cell death in flower petals. Plant Mol Biol 44:303–318
  37. Sambrook J, Russell DW (2001) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
  38. Sanchez AM, Bosch M, Bots M, Nieuwland J, Feron R, Mariani C (2004) Pistil factors controlling pollination. Plant Cell 16:S98–S106
  39. Sasabe M, Toyoda K, Shiraishi T, Inagaki Y, Ichinose Y (2002) cDNA cloning and characterization of tobacco ABC transporter: NtPDR1 is a novel elicitor-responsive gene. FEBS Lett 518:164–168
  40. Silva NF, Goring DR (2001) Mechanisms of self-incompatibility in flowering plants. Cell Mol Life Sci 58:1988–2007
  41. Smart CC, Fleming AJ (1996) Hormonal and environmental regulation of a plant PDR5-like ABC transporter. J Biol Chem 271:19351–19357
  42. Stein M, Dittgen J, Sanchez-Rodriguez C, Hou BH, Molina A, Schulze-Lefert P, Lipka V, Somerville S (2006) Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration. Plant Cell 18:731–746
  43. Stukkens Y, Bultreys A, Grec S, Trombik T, Vanham D, Boutry M (2005) NpPDR1, a pleiotropic drug resistance-type ATP-binding cassette transporter from Nicotiana plumbaginifolia, plays a major role in plant pathogen defense. Plant Physiol 139:341–352
  44. Swanson R, Edlund AF, Preuss D (2004) Species specificity in pollen-pistil interactions. Annu Rev Genet 38:793–818
  45. Takayama S, Isogai A (2005) Self-incompatibility in plants. Annu Rev Plant Biol 56:467–489
  46. Theodoulou FL (2000) Plant ABC transporters. Biochim Biophys Acta 1465:79–103
  47. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
  48. Tudzynski P, Scheffer J (2004) Claviceps purpurea: molecular aspects of a unique pathogenic lifestyle. Mol Plant Pathol 5:377–388
  49. van den Brule S, Muller A, Fleming AJ, Smart CC (2002) The ABC transporter SpTUR2 confers resistance to the antifungal diterpene sclareol. Plant J 30:649–662
  50. Van den Brûle S, Smart CC (2002) The plant PDR family of ABC transporters. Planta 216:95–106
  51. Wesley SV, Helliwell CA, Smith NA, Wang M, Rouse DT, Liu Q, Gooding PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, Green AG, Waterhouse PM (2001) Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J 27:581–590
  52. Wheeler MJ, Franklin-Tong VE, Franklin FCH (2001) The molecular and genetic basis of pollen-pistil interactions. New Phytol 151:565–584
  53. Wu HM, Wong E, Ogdahl J, Cheung AY (2000) A pollen tube growth-promoting arabinogalactan protein from Nicotiana alata is similar to the tobacco TTS protein. Plant J 22:165–176
  54. Zimmermann P, Hirsch-Hoffmann M, Hennig L, Gruissem W (2004) GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox. Plant Physiol 136:2621–2632
  55. Jasisnki M, Ducos E, Martionia E, Boutry M (2003) The ATP-Binding Cassette transporters: structure, function and gene family comparison between rice and Arabidopsis. Plant Physiol 131:1169–1177
Bibliographic reference Trombik, Tomasz ; Jasinski, Michal ; Crouzet, Jérome ; Boutry, Marc. Identification of a cluster IV pleiotropic drug resistance transporter gene expressed in the style of Nicotiana plumbaginifolia.. In: Plant molecular biology, Vol. 66, no. 1-2, p. 165-75 (2008)
Permanent URL http://hdl.handle.net/2078.1/11200