User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Double protein functionalized poly-ε-caprolactone surfaces: in depth ToF–SIMS and XPS characterization

Primary tabs

Desmet, T. [Ghent University, Krijgslaan 281 S4 Bis, 9000 Ghent, Belgium] Poleunis, Claude [UCL] Delcorte, Arnaud [UCL] Dubruel, Peter [Ghent University, Krijgslaan 281 S4 Bis, 9000 Ghent, Belgium]

In biomaterial research, great attention has focussed on the immobilization of biomolecules with the aim to increase cell-adhesive properties of materials. Many different strategies can be applied. In previously published work, our group focussed on the treatment of poly-ecaprolactone (PCL) films by an Ar-plasma, followed by the grafting of 2-aminoethyl methacrylate (AEMA) under UV-irradiation. The functional groups introduced, enabled the subsequent covalent immobilisation of gelatin. The obtained coating was finally applied for the physisorption of fibronectin. The successful PCL surface functionalization was preliminary confirmed using XPS, wettability studies, AFM and SEM. In the present article, we report on an in-depth characterization of the materials developed using ToF–SIMS and XPS analysis. The homogeneous AEMA grafting and the subsequent protein coating steps could be confirmed by both XPS and ToF–SIMS. Using ToF–SIMS, it was possible to demonstrate the presence of polymethacrylates on the surface. From peak deconvoluted XPS results (C- and N-peak), the presence of proteins could be confirmed. Using ToF–SIMS, different positive ions, correlating to specific amino-acids could be identified. Importantly, the gelatin and the fibronectin coatings could be qualitatively distinguished. Interestingly for biomedical applications, ethylene oxide sterilization did not affect the surface chemical composition. This research clearly demonstrates the complementarities of XPS and ToF–SIMS in biomedical surface modification research.

Document type Article de périodique (Journal article) – Article de recherche
Access type Accès restreint
Publication date 2012
Language Anglais
Journal information "Journal of Materials Science: Materials in Medicine" - Vol. 23, no. 2, p. 293-305
Peer reviewed yes
Publisher Springer New York LLC ((United States) New York)
issn 0957-4530
Publication status Publié
Affiliations Ghent University, Krijgslaan 281 S4 Bis, 9000 Ghent, Belgium - Polymer Chemistry & Biomaterials Research Group
UCL - SST/IMCN/BSMA - Bio and soft matter
Links
  1. Desmet T, Schacht E, Dubruel P. Rapid prototyping as an elegant production tool for polymeric tissue engineering scaffolds: a review. In: Barnes SJ, Harris LP, editors. Tissue engineering: roles, materials and applications. New York: Nova Science Publishers, Inc.; 2008; p. 141–189.
  2. Desmet Tim, Morent Rino, De Geyter Nathalie, Leys Christophe, Schacht Etienne, Dubruel Peter, Nonthermal Plasma Technology as a Versatile Strategy for Polymeric Biomaterials Surface Modification: A Review, 10.1021/bm900186s
  3. Goddard J.M., Hotchkiss J.H., Polymer surface modification for the attachment of bioactive compounds, 10.1016/j.progpolymsci.2007.04.002
  4. Jagielski J., Turos A., Bielinski D., Abdul-Kader A.M., Piatkowska A., Ion-beam modified polymers for biomedical applications, 10.1016/j.nimb.2007.03.021
  5. Marletta Giovanni, Ciapetti Gabriela, Satriano Cristina, Pagani Stefania, Baldini Nicola, The effect of irradiation modification and RGD sequence adsorption on the response of human osteoblasts to polycaprolactone, 10.1016/j.biomaterials.2004.11.047
  6. Sioshansi Piran, Tobin Eric J., Surface treatment of biomaterials by ion beam processes, 10.1016/0257-8972(95)02838-2
  7. Zhu Aiping, Zhao Feng, Ma Teng, Photo-initiated grafting of gelatin/N-maleic acyl-chitosan to enhance endothelial cell adhesion, proliferation and function on PLA surface, 10.1016/j.actbio.2009.02.016
  8. Truica-Marasescu Florina, Wertheimer Michael R., Vacuum-Ultraviolet Photopolymerisation of Amine-Rich Thin Films, 10.1002/macp.200800089
  9. Farquet Patrick, Padeste Celestino, Solak Harun H., Gürsel Selmiye Alkan, Scherer Günther G., Wokaun Alexander, EUV lithographic radiation grafting of thermo-responsive hydrogel nanostructures, 10.1016/j.nimb.2007.08.070
  10. Wirsén Anders, Sun Hui, Albertsson Ann-Christine, Solvent-Free Vapor-Phase Photografting of Acrylamide onto Poly(ethylene terephthalate), 10.1021/bm050169a
  11. Bullett Nial A., Bullett Diane P., Truica-Marasescu Florina-Elena, Lerouge Sophie, Mwale Fackson, Wertheimer Michael R., Polymer surface micropatterning by plasma and VUV-photochemical modification for controlled cell culture, 10.1016/j.apsusc.2004.02.058
  12. Mathieson I., Bradley R.H., Improved adhesion to polymers by UV/ozone surface oxidation, 10.1016/0143-7496(96)88482-x
  13. Siow Kim Shyong, Britcher Leanne, Kumar Sunil, Griesser Hans J., Plasma Methods for the Generation of Chemically Reactive Surfaces for Biomolecule Immobilization and Cell Colonization - A Review, 10.1002/ppap.200600021
  14. Desmet Tim, Billiet T., Berneel Elke, Cornelissen Ria, Schaubroeck David, Schacht Etienne, Dubruel Peter, Post-Plasma Grafting of AEMA as a Versatile Tool to Biofunctionalise Polyesters for Tissue Engineering, 10.1002/mabi.201000147
  15. Chim H., Hutmacher D.W., Chou A.M., Oliveira A.L., Reis R.L., Lim T.C., Schantz J.-T., A comparative analysis of scaffold material modifications for load-bearing applications in bone tissue engineering, 10.1016/j.ijom.2006.03.024
  16. Hsu Shan-hui, Yen Hung-Jen, Tseng Ching-Shiow, Cheng Chia-Sheng, Tsai Ching-Lin, Evaluation of the growth of chondrocytes and osteoblasts seeded into precision scaffolds fabricated by fused deposition manufacturing, 10.1002/jbm.b.30626
  17. Olah Laszlo, Filipczak Katarzyna, Jaegermann Zbigniew, Czigany Tibor, Borbas Lajos, Sosnowski Stanislaw, Ulanski Piotr, Rosiak Janusz M., Synthesis, structural and mechanical properties of porous polymeric scaffolds for bone tissue regeneration based on neat poly(ɛ-caprolactone) and its composites with calcium carbonate, 10.1002/pat.768
  18. Perale G., Pertici G., Giordano C., Daniele F., Masi M., Maccagnan S., Nondegradative microextrusion of resorbable polyesters for pharmaceutical and biomedical applications: The cases of poly-lactic-acid and poly-caprolactone, 10.1002/app.27875
  19. Savarino L., Baldini N., Greco M., Capitani O., Pinna S., Valentini S., Lombardo B., Esposito M.T., Pastore L., Ambrosio L., The performance of poly-ε-caprolactone scaffolds in a rabbit femur model with and without autologous stromal cells and BMP4, 10.1016/j.biomaterials.2007.03.011
  20. Shor Lauren, Güçeri Selçuk, Wen Xuejun, Gandhi Milind, Sun Wei, Fabrication of three-dimensional polycaprolactone/hydroxyapatite tissue scaffolds and osteoblast-scaffold interactions in vitro, 10.1016/j.biomaterials.2007.08.018
  21. Wang F., Shor L., Darling A., Khalil S., Sun W., Güçeri S., Lau A., Precision extruding deposition and characterization of cellular poly‐ε‐caprolactone tissue scaffolds, 10.1108/13552540410512525
  22. Zein Iwan, Hutmacher Dietmar W., Tan Kim Cheng, Teoh Swee Hin, Fused deposition modeling of novel scaffold architectures for tissue engineering applications, 10.1016/s0142-9612(01)00232-0
  23. Hutmacher Dietmar W., Schantz Thorsten, Zein Iwan, Ng Kee Woei, Teoh Swee Hin, Tan Kim Cheng, Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling, 10.1002/1097-4636(200105)55:2<203::aid-jbm1007>3.0.co;2-7
  24. Cheng Ziyuan, Teoh Swee-Hin, Surface modification of ultra thin poly (ε-caprolactone) films using acrylic acid and collagen, 10.1016/j.biomaterials.2003.08.038
  25. Amato Ilaria, Ciapetti Gabriela, Pagani Stefania, Marletta Giovanni, Satriano Cristina, Baldini Nicola, Granchi Donatella, Expression of cell adhesion receptors in human osteoblasts cultured on biofunctionalized poly-(ε-caprolactone) surfaces, 10.1016/j.biomaterials.2007.04.038
  26. Gabriel Matthias, van Nieuw Amerongen Geerten P., van Hinsbergh Victor W. M., van Nieuw Amerongen Arie V., Zentner Andrej, Direct grafting of RGD-motif-containing peptide on the surface of polycaprolactone films, 10.1163/156856206776986288
  27. Ma Zuwei, He Wei, Yong Thomas, Ramakrishna S., Grafting of Gelatin on Electrospun Poly(caprolactone) Nanofibers to Improve Endothelial Cell Spreading and Proliferation and to Control Cell Orientation, 10.1089/ten.2005.11.1149
  28. Marletta G., Ciapetti G., Satriano C., Perut F., Salerno M., Baldini N., Improved osteogenic differentiation of human marrow stromal cells cultured on ion-induced chemically structured poly-ε-caprolactone, 10.1016/j.biomaterials.2006.10.027
  29. Tiaw K.S., Goh S.W., Hong M., Wang Z., Lan B., Teoh S.H., Laser surface modification of poly(ε-caprolactone) (PCL) membrane for tissue engineering applications, 10.1016/j.biomaterials.2004.03.010
  30. Wirsén A., Sun H., Emilsson L., Albertsson A.-C., Solvent Free Vapor Phase Photografting of Maleic Anhydride onto Poly(ethylene terephthalate) and Surface Coupling of Fluorinated Probes, PEG, and an RGD-Peptide, 10.1021/bm050170+
  31. Zhu Yabin, Gao Changyou, Liu Xingyu, Shen Jiacong, Surface Modification of Polycaprolactone Membrane via Aminolysis and Biomacromolecule Immobilization for Promoting Cytocompatibility of Human Endothelial Cells, 10.1021/bm020074y
  32. Yu T.Tina, Shoichet Molly S., Guided cell adhesion and outgrowth in peptide-modified channels for neural tissue engineering, 10.1016/j.biomaterials.2004.05.012
  33. Yang Qian, Xu Zhi-Kang, Hu Meng-Xin, Li Jun-Jie, Wu Jian, Novel Sequence for Generating Glycopolymer Tethered on a Membrane Surface, 10.1021/la051797g
  34. Yang Q, Xu ZK, Ulbricht M. Surface modification of polypropylene microporous membrane by the immobilization of dextran. Chem J Chin Univ-Chin. 2005;26(1):189–91.
  35. YU H, HE J, LIU L, HE X, GU J, WEI X, Photoinduced graft polymerization to improve antifouling characteristics of an SMBR, 10.1016/j.memsci.2007.06.061
  36. Thompson Kate L., Read Elizabeth S., Armes Steven P., Chemical degradation of poly(2-aminoethyl methacrylate), 10.1016/j.polymdegradstab.2008.05.013
  37. Van Vlierberghe Sandra, Cnudde Veerle, Dubruel Peter, Masschaele Bert, Cosijns An, De Paepe Ilse, Jacobs Patric J. S., Van Hoorebeke Luc, Remon Jean Paul, Schacht Etienne, Porous Gelatin Hydrogels:  1. Cryogenic Formation and Structure Analysis, 10.1021/bm060684o
  38. Ulubayram Kezban, Eroglu Inci, Hasirci Nesrin, Gelatin Microspheres and Sponges for Delivery of Macromolecules, 10.1177/0885328202016003178
  39. Jingrun Ren, Jin Wang, Hong Sun, Nan Huang, Surface modification of polyethylene terephthalate with albumin and gelatin for improvement of anticoagulation and endothelialization, 10.1016/j.apsusc.2008.06.181
  40. Shin Young Min, Kim Kyung-Soo, Lim Youn Mook, Nho Young Chang, Shin Heungsoo, Modulation of Spreading, Proliferation, and Differentiation of Human Mesenchymal Stem Cells on Gelatin-Immobilized Poly(l-lactide-co-ϵ-caprolactone) Substrates, 10.1021/bm701410g
  41. Zhu Yabin, Gao Changyou, He Tao, Shen Jiacong, Endothelium regeneration on luminal surface of polyurethane vascular scaffold modified with diamine and covalently grafted with gelatin, 10.1016/s0142-9612(03)00549-0
  42. Zhu Yabin, Gao Changyou, Liu Xingyu, He Tao, Shen Jiacong, Immobilization of Biomacromolecules onto Aminolyzed Poly(L-lactic acid) toward Acceleration of Endothelium Regeneration, 10.1089/107632704322791691
  43. Zhu Yabin, Gao Changyou, Shen Jiacong, Surface modification of polycaprolactone with poly(methacrylic acid) and gelatin covalent immobilization for promoting its cytocompatibility, 10.1016/s0142-9612(02)00247-8
  44. Van Vlierberghe Sandra, Vanderleyden Els, Dubruel Peter, De Vos Filip, Schacht Etienne, Affinity Study of Novel Gelatin Cell Carriers for Fibronectin, 10.1002/mabi.200900043
  45. Biosurface Organic Chemistry: Interfacial Chemical Reactions for Applications to Nanobiotechnology and Biomedical Sciences, 10.5012/bkcs.2005.26.3.361
  46. Vogel, V. Fibronectin in a surface-adsorbed state. In: Horbett TA, Brash JL, editors. Proteins at interfaces II, fundamentals and applications. Washington: American Chemical Society; 1995.
  47. Altankov G., Thom V., Groth T., Jankova K., Jonsson G., Ulbricht M., Modulating the biocompatibility of polymer surfaces with poly(ethylene glycol): Effect of fibronectin, 10.1002/1097-4636(200010)52:1<219::aid-jbm28>3.0.co;2-f
  48. Badylak S, Endothelial cell adherence to small intestinal submucosa: an acellular bioscaffold, 10.1016/s0142-9612(99)00156-8
  49. Detrait E., Lhoest J.-B., Bertrand P., van den Bosch de Aguilar Ph., Fibronectin-pluronic coadsorption on a polystyrene surface with increasing hydrophobicity: Relationship to cell adhesion, 10.1002/(sici)1097-4636(19990615)45:4<404::aid-jbm16>3.0.co;2-4
  50. Suzuki M., Kishida A., Iwata H., Ikada Y., Graft copolymerization of acrylamide onto a polyethylene surface pretreated with glow discharge, 10.1021/ma00161a005
  51. Chong M.S.K., Lee C.N., Teoh S.H., Characterization of smooth muscle cells on poly(ε-caprolactone) films, 10.1016/j.msec.2006.03.008
  52. Genet M, Dupont-Gillain C, Rouxhet P. XPS analysis of biosystems and biomaterials. In: Matijevi′c E, editor. Medical applications of colloids. New York: Springer; 2008, p. 177–307.
  53. Briggs D, Surface analysis of polymers by XPS and static SIMS. Cambridge: Cambridge University Press; 1998, p xiii+198.
  54. Canavan Heather E., Graham Daniel J., Cheng Xuanhong, Ratner Buddy D., Castner David G., Comparison of Native Extracellular Matrix with Adsorbed Protein Films Using Secondary Ion Mass Spectrometry†, 10.1021/la062330o
  55. Vickerman JC, Briggs D, Henderson A. The Wiley static SIMS library. Chichester: Wiley; 1996, p. v.
Bibliographic reference Desmet, T. ; Poleunis, Claude ; Delcorte, Arnaud ; Dubruel, Peter. Double protein functionalized poly-ε-caprolactone surfaces: in depth ToF–SIMS and XPS characterization. In: Journal of Materials Science: Materials in Medicine, Vol. 23, no. 2, p. 293-305
Permanent URL http://hdl.handle.net/2078.1/107509