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Selection of allosteric beta-lactamase mutants featuring an activity regulation by transition metal ions.

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Mathonet, Pascale Barrios, Humberto Soumillion, Patrice [UCL] Fastrez, Jacques [UCL]

Libraries of phage-displayed beta-lactamase mutants in which up to three loops have been engineered by genetic introduction of random peptide sequences or by randomization of the wild-type sequence have been submitted to selection protocols designed to find mutants in which binding of transition metal ions to the engineered secondary binding site leads to significant effects on the enzymatic activity. A double-selection protocol was applied: The phage-displayed libraries were first selected for transition metal ions affinity by panning on IMAC support, then a second selection step was applied to isolate mutants that have retained significant catalytic activity. The analysis of the kinetic properties of mutants in the presence of nickel, copper, or zinc ions allowed isolation of a few mutants whose activity was either enhanced or inhibited by factors up to three and >10, respectively, in a metal-specific manner. A remarkable mutant exhibiting differential allosteric regulation depending on the metal was found. Its activity was activated by nickel ion binding, inhibited by cupric ion binding, and nearly unaffected by zinc ions. These observations point to an interesting potential for up- or down-regulation of activity within a monomeric enzyme by binding to an "allosteric site" relatively remote from the active site.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès libre
Publication date 2006
Language Anglais
Journal information "Protein science : a publication of the Protein Society" - Vol. 15, no. 10, p. 2335-43 (2006)
Peer reviewed yes
issn 0961-8368
Publication status Publié
Affiliation UCL - SC/CHIM - Département de chimie
MESH Subject Allosteric Regulation - genetics ; Allosteric Site - genetics ; Copper - pharmacology ; Kinetics ; Mutation ; Nickel - pharmacology ; Peptide Library ; Protein Engineering ; Transition Elements - pharmacology ; Zinc - pharmacology ; beta-Lactamases - chemistry - genetics - metabolism
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Bibliographic reference Mathonet, Pascale ; Barrios, Humberto ; Soumillion, Patrice ; Fastrez, Jacques. Selection of allosteric beta-lactamase mutants featuring an activity regulation by transition metal ions.. In: Protein science : a publication of the Protein Society, Vol. 15, no. 10, p. 2335-43 (2006)
Permanent URL http://hdl.handle.net/2078.1/10703