User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Identification of a Nicotiana plumbaginifolia plasma membrane H(+)-ATPase gene expressed in the pollen tube.

Primary tabs

Lefebvre, Benoit Arango, Miguel Oufattole, Mohammed Crouzet, Jérôme [UCL] Purnelle, Bénédicte [UCL] Boutry, Marc [UCL]

In Nicotiana plumbaginifolia, plasma membrane H(+)-ATPases (PMAs) are encoded by a gene family of nine members. Here, we report on the characterization of a new isogene, NpPMA5 (belonging to subfamily IV), and the determination of its expression pattern using the beta-glucuronidase (gusA) reporter gene. pNpPMA5-gusA was expressed in cotyledons, in vascular tissues of the stem (mainly in nodal zones), and in the flower and fruit. In the flower, high expression was found in the pollen tube after in vitro or in vivo germination. Northern blotting analysis confirmed that NpPMA5 was expressed in the pollen tube contrary to NpPMA2 (subfamily I) or NpPMA4 (subfamily II), two genes highly expressed in other tissues. The subcellular localization of PM H(+)-ATPase in the pollen tube was analyzed by immunocytodecoration. As expected, this enzyme was localized to the plasma membrane. However, neither the tip nor the base of the pollen tube was labeled, showing an asymmetrical distribution of this enzyme. This observation supports the hypothesis that the PM H(+)-ATPase is involved in creating the pH gradient that is observed along the pollen tube and is implicated in cell elongation. Compared to other plant PM H(+)-ATPases, the C-terminal region of NpPMA5 is shorter by 26 amino acid residues and is modified in the last 6 residues, due to a sequence rearrangement, which was also found in the orthologous gene of Nicotiana glutinosa, a Nicotiana species distant from N. plumbaginifolia and Petunia hybrida and Lycopersicon esculentum, other Solanacae species. This modification alters part of the PM H(+)-ATPase regulatory domain and raises the question whether this isoform is still regulated.

Document type Article de périodique (Journal article) – Article de recherche – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 2005
Language Anglais
Journal information "Plant molecular biology" - Vol. 58, no. 6, p. 775-87 (2005)
Peer reviewed yes
issn 0167-4412
Publication status Publié
Affiliation UCL - AGRO/CABI - Département de chimie appliquée et des bio-industries
MESH Subject Base Sequence ; Cell Membrane - enzymology ; Flowers - cytology - enzymology - genetics ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Molecular Sequence Data ; Organ Specificity ; Phylogeny ; Proton-Translocating ATPases - genetics - metabolism ; Sequence Alignment ; Sequence Homology, Nucleic Acid ; Tobacco - cytology - enzymology - genetics
Links
  1. M. Arango, Planta, 216, 355 (2003)
  2. K.B. Axelsen, Biochemistry, 38, 7227 (1999)
  3. I. Baxter, Plant Physiol., 132, 618 (2003)
  4. L. Camoni, J. Biol. Chem., 275, 9919 (2000)
  5. S. Dambly, J. Biol. Chem., 276, 7017 (2001)
  6. A. Kerchove d’Exaerde de, J. Biol. Chem., 270, 23828 (1995)
  7. N.D. DeWitt, Plant J., 1, 121 (1991)
  8. J.A. Feijo, Protoplasma, 187, 155 (1995)
  9. J.A. Feijo, J. Cell Biol., 144, 483 (1999)
  10. J.A. Feijo, Bioessays, 23, 86 (2001)
  11. M.D. Fricker, J. Cell Sci., 110, 1729 (1997)
  12. A.T. Fuglsang, J. Biol. Chem., 274, 36774 (1999)
  13. T.H. Goodspeed, The Genus NicotianaWaltham (1954)
  14. T.L. Holdaway-Clarke, New Phytol., 159, 539 (2003)
  15. R.B. Horsch, Proc. Natl. Acad. Sci. USA, 83, 2571 (1986)
  16. B. Lefebvre, Prog. Nucleic Acid Res., 74, 203 (2003)
  17. B. Lefebvre, Plant Cell, 16, 1772 (2004)
  18. H. Luo, Plant Physiol., 119, 627 (1999)
  19. MALIGA P., SZ.-BREZNOVITS ÁGNES, MÁRTON L., Streptomycin-resistant Plants from Callus Culture of Haploid Tobacco, 10.1038/newbio244029a0
  20. J.P. Mascarenhas, Plant J., 2, 405 (1992)
  21. O. Maudoux, J. Biol. Chem., 275, 17762 (2000)
  22. M.A. Messerli, Plant J., 16, 87 (1998)
  23. B. Michelet, Plant Cell, 6, 1375 (1994)
  24. L. Moriau, Plant J., 19, 31 (1999)
  25. P. Morsomme, Embo J., 15, 5513 (1996)
  26. G. Obermeyer, Protoplasma, 171, 55 (1992)
  27. G. Obermeyer, Physiol. Plant., 98, 281 (1996)
  28. H. Ochman, Genetics, 120, 621 (1988)
  29. A. Olson, Plant Physiol., 118, 551 (1998)
  30. M. Oufattole, Planta, 210, 715 (2000)
  31. M.G. Palmgren, J Biol. Chem., 266, 20470 (1991)
  32. M.G. Palmgren, Annu. Rev. Plant Physiol. Plant Mol. Biol., 52, 817 (2001)
  33. R.M. Parton, J. Cell Sci., 110, 1187 (1997)
  34. H. Pertl, Planta, 213, 132 (2001)
  35. B. Regenberg, Plant Cell, 7, 1655 (1995)
  36. W.R. Robertson, Genetics, 168, 16677 (2004)
  37. M.P. Rodriguez-Rosales, Plant Physiol. Biochem., 27, 723 (1989)
  38. J.D. Thompson, Nucleic Acids Res., 22, 4673 (1994)
  39. S. Uknes, Plant Cell, 5, 159 (1993)
Bibliographic reference Lefebvre, Benoit ; Arango, Miguel ; Oufattole, Mohammed ; Crouzet, Jérôme ; Purnelle, Bénédicte ; et. al. Identification of a Nicotiana plumbaginifolia plasma membrane H(+)-ATPase gene expressed in the pollen tube.. In: Plant molecular biology, Vol. 58, no. 6, p. 775-87 (2005)
Permanent URL http://hdl.handle.net/2078.1/10362