User menu

Accès à distance ? S'identifier sur le proxy UCLouvain

Production of [2-11C]thymidine for quantification of cellular proliferation with PET.

Primary tabs

Vander Borght, Thierry [UCL] Labar, Daniel [UCL] Pauwels, Stanislas [UCL] Lambotte, Luc [UCL]

A three-step synthesis of [2-11C]thymidine, using [11C]urea as precursor, is described. [2-11C]Thymine was obtained by cyclization of [11C]urea and diethyl beta-methylmalate in fuming sulfuric acid. After purification of the reaction mixture, [2-11C]thymine and 2'-deoxyribose-1-phosphate were incubated in the presence of thymidine phosphorylase to form [2-11C]thymidine. The whole synthesis procedure, including purification and pharmaceutical package, was achieved within 60 min with a decay corrected yield of 30-35%.

Document type Article de périodique (Journal article) – Journal Article, Research Support, Non-U.S. Gov't
Access type Accès restreint
Publication date 1991
Journal information "International Journal of Radiation Applications and Instrumentation. Part A: Applied Radiation and Isotopes" - Vol. 42, no. 1, p. 103-104 (1991)
Publisher Pergamon
issn 0883-2889
Publication status Publié
Affiliations UCL - MD/MINT - Département de médecine interne
UCL - MD/CHIR - Département de chirurgie
MESH Subject Carbon Radioisotopes - diagnostic use ; Cell Division ; Thymine - metabolism ; Tomography, Emission-Computed
Links
Bibliographic reference Vander Borght, Thierry ; Labar, Daniel ; Pauwels, Stanislas ; Lambotte, Luc. Production of [2-11C]thymidine for quantification of cellular proliferation with PET.. In: International Journal of Radiation Applications and Instrumentation. Part A: Applied Radiation and Isotopes, Vol. 42, no. 1, p. 103-104 (1991)
Permanent URL http://hdl.handle.net/2078.1/13754